Finotti Alessia, Gasparello Jessica, Lampronti Ilaria, Cosenza Lucia Carmela, Maconi Giovanni, Matarese Vincenzo, Gentili Valentina, Di Luca Dario, Gambari Roberto, Caselli Michele
Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
Department of Gastroenterology, Sacco Hospital, University of Milan, Milan, Italy.
BMJ Open Gastroenterol. 2017 Dec 4;4(1):e000172. doi: 10.1136/bmjgast-2017-000172. eCollection 2017.
Segmented filamentous bacteria (SFB) have been detected in a wide range of different animal. Recently, the presence of SFB-like bacteria was shown in biopsies of the terminal ileum and ileocecal valve of both patients with ulcerative colitis and control subjects. The aim of this study was to verify whether PCR methods could be used for the detection of SFB in biopsy of patients with ulcerative colitis and its relationships with the disease stage.
PCR methods were used to identify SFB in biopsies from the terminal ileum of patients with ulcerative colitis, showing that this approach represents a useful tool for the detection of SFB presence and analysis of the bacterial load.
Our analysis detected SFB in all faecal samples of children at the time of weaning, and also show that putative SFB sequences are present in both patients with ulcerative colitis and control subjects. Results obtained using real-time quantitative PCR analysis confirm the presence of putative SFB sequences in samples from the terminal ileum of patients with ulcerative colitis and in control subjects.
The presence of putative SFB sequence in both patients with ulcerative colitis and control subject suggests that SFB cannot be considered as being uniquely associated with the disease. The second conclusion is that among the patients with ulcerative colitis, a tendency does exist for active disease samples to show higher SFB load, opening new perspectives about possible identification and pharmacological manipulation of SFB-mediated processes for new therapeutic strategy.
已在多种不同动物中检测到分节丝状菌（SFB）。最近，在溃疡性结肠炎患者和对照受试者的回肠末端及回盲瓣活检组织中均发现了类似SFB的细菌。本研究的目的是验证PCR方法是否可用于检测溃疡性结肠炎患者活检组织中的SFB及其与疾病分期的关系。
采用PCR方法鉴定溃疡性结肠炎患者回肠末端活检组织中的SFB，结果表明该方法是检测SFB存在及分析细菌载量的有用工具。
我们的分析在断奶时儿童的所有粪便样本中均检测到SFB，并且还表明溃疡性结肠炎患者和对照受试者中均存在假定的SFB序列。使用实时定量PCR分析获得的结果证实了溃疡性结肠炎患者回肠末端样本和对照受试者中存在假定的SFB序列。
溃疡性结肠炎患者和对照受试者中均存在假定的SFB序列，这表明SFB不能被认为仅与该疾病相关。第二个结论是，在溃疡性结肠炎患者中，活动期疾病样本确实有显示出更高SFB载量的趋势，这为新治疗策略中SFB介导过程的可能鉴定和药物调控开辟了新的前景。
