Yuan Shengliang, Duan Zhihong, Lu Yingnian, Ma Xiaoli, Wang Sheng
Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Zhanjiang, 524023 China.
Affiliated Hospital, Guangdong Medical University, Zhanjiang, 524023 China.
3 Biotech. 2018 Jan;8(1):8. doi: 10.1007/s13205-017-1037-6. Epub 2017 Dec 11.
is mainly used as a raw material source for agar industry, and its extract is rich in natural pigments with antioxidant activities. In this study, a solvent reflux extraction method for decolorization of has been developed in agar production. The extraction conditions were optimized as follows: solvent-to-material ratio, 50:1; ethanol concentration, 70%; number of extractions, 3; extraction time, 0.5 h, under which the total antioxidant yield of the extract reached 2.89 ± 0.88 mg/g dried seaweeds. Their IC values of DPPH radical scavenging activity, hydroxyl radical scavenging activity and superoxide anion scavenging activity were 21.91 ± 1.8 mg/L, 40.59 ± 1.5 mg/L and 160.87 ± 2.8 mg/L, respectively. Further isolation and spectroscopic analysis of natural pigments suggested the strong antioxidant capacities were attributed to chlorophyll derivatives. The results indicate that the decolorization process was able to improve the agar quality, and the extract containing lots of natural pigments had antioxidant activities which may be used in functional food and cosmetics.
主要用作琼脂工业的原料来源，其提取物富含具有抗氧化活性的天然色素。在本研究中，开发了一种用于琼脂生产中脱色的溶剂回流提取方法。提取条件优化如下：料液比50:1；乙醇浓度70%；提取次数3次；提取时间0.5小时，在此条件下提取物的总抗氧化产率达到2.89±0.88毫克/克干海藻。其对DPPH自由基清除活性、羟基自由基清除活性和超氧阴离子清除活性的IC值分别为21.91±1.8毫克/升、40.59±1.5毫克/升和160.87±2.8毫克/升。对天然色素的进一步分离和光谱分析表明，其强大的抗氧化能力归因于叶绿素衍生物。结果表明，脱色过程能够提高琼脂质量，含有大量天然色素的提取物具有抗氧化活性，可用于功能性食品和化妆品。
