Instituto Cajal, CSIC, Madrid, Spain.
Laboratorio Cajal de Circuitos Corticales (CTB), Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223, Madrid, Spain.
Brain Struct Funct. 2018 May;223(4):1881-1895. doi: 10.1007/s00429-017-1596-7. Epub 2017 Dec 19.
Mammalian hibernation proceeds alongside a wide range of complex brain adaptive changes that appear to protect the brain from extreme hypoxia and hypothermia. Using immunofluorescence, confocal microscopy, quantitative analysis methods and intracellular injections, we have characterized microglia morphological changes that occur in the neocortex and hippocampus of the Syrian hamster during hibernation. In euthermic hamsters, microglial cells showed the typical ramified/resting morphology with multiple long, thin and highly-branched processes homogeneously immunostained for Iba-1. However, during torpor, microglial cell process numbers increase significantly accompanied by a shortening of the Iba-1 immunoreactive processes, which show a fragmented appearance. Adaptative changes of microglial cells during torpor coursed with no expression of microglial cell activation markers. We discuss the possibility that these morphological changes may contribute to neuronal damage prevention during hibernation.
哺乳动物的冬眠伴随着广泛的复杂的大脑适应性变化，这些变化似乎保护大脑免受极端缺氧和低温的影响。使用免疫荧光、共聚焦显微镜、定量分析方法和细胞内注射，我们描述了叙利亚仓鼠在冬眠期间新皮层和海马体中小胶质细胞形态的变化。在体温正常的仓鼠中，小胶质细胞表现出典型的分支/静止形态，用 Iba-1 均匀免疫染色，具有多个长、细和高度分支的过程。然而，在蛰伏期间，小胶质细胞的过程数量显著增加，伴随着 Iba-1 免疫反应过程的缩短，呈现出碎片化的外观。蛰伏期间小胶质细胞的适应性变化伴随着小胶质细胞激活标志物的表达缺失。我们讨论了这些形态变化可能有助于防止冬眠期间神经元损伤的可能性。
