College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju, 52828, Korea.
College of Pharmacy, Chungbuk National University, Cheongju, 28644, Korea.
J Nat Med. 2018 Mar;72(2):409-423. doi: 10.1007/s11418-017-1164-8. Epub 2017 Dec 19.
The present study was designed to establish quality control parameters for pharmacognostic evaluation and differentiation of eight locally derived Boehmeria species, B. gracilis, B. nivea, B. pannosa, B. platanifolia, B. quelpaertensis, B. spicata, B. splitgerbera, B. tricuspis, and two varieties named B. japonica var. longispica, B. nivea var. concolor, which have been utilized as the folk medicine, 'Mo-Si-Pool' in Korea. Although the outer morphological study of these species had been reported, there is no pharmacognostical description yet. Therefore, inner morphological evaluation on leaf midrib, petiole and stem of eight Boehmeria species and two varieties was accomplished along with preliminary phytochemical analysis by HPLC-DAD profiling. The microscopic data showed discriminative inner morphological characteristics such as collenchyma cell layer, thickness of cortex and xylem, frequency of druse and hairs, and number of vascular bundles. The HPLC profiles exhibited more than four characteristic peaks. The molecular ions of the four peaks (1-4) were tentatively identified by ESI-MS, and their structures were identified by NMR spectroscopy to be the flavonoids, rutin (1), isoquercetin (2) and kaempferol-3-O-rutinoside (3), and a phenanthroquinolizidine alkaloid, (-)-cryptopleurine (4). While compounds 1 and 2 were detected in all samples, compound 4 was determined only in B. japonica var. longispica, B. pannosa and B. quelpaertensis and B. splitgerbera. These findings provide the initial scientific criteria for proper identification and establishment of standards for use of Boehmeria species in traditional medicine.
本研究旨在为 8 种当地衍生的苎麻属物种（Boehmeria gracilis、B. nivea、B. pannosa、B. platanifolia、B. quelpaertensis、B. spicata、B. splitgerbera、B. tricuspis 和两个变种，B. japonica var. longispica 和 B. nivea var. concolor）的生药学评价和鉴别建立质量控制参数，这些物种被用作韩国的民间药物“Mo-Si-Pool”。尽管这些物种的外部形态学研究已经有报道，但目前还没有生药学描述。因此，我们对 8 种苎麻属物种和 2 个变种的叶片中脉、叶柄和茎进行了内部形态学评估，并结合 HPLC-DAD 分析进行了初步的植物化学分析。微观数据显示出具有鉴别性的内部形态特征，如厚角组织细胞层、皮层和木质部的厚度、晶簇和毛发的频率以及维管束的数量。HPLC 图谱显示出超过四个特征峰。通过 ESI-MS 对四个峰（1-4）的分子离子进行了初步鉴定，并通过 NMR 光谱鉴定了它们的结构，分别为黄酮类化合物芦丁（1）、异槲皮苷（2）和山奈酚-3-O-芦丁糖苷（3）以及一种菲并喹啉里西啶生物碱（-）-隐石松碱（4）。虽然化合物 1 和 2 存在于所有样品中，但化合物 4 仅存在于 B. japonica var. longispica、B. pannosa 和 B. quelpaertensis 以及 B. splitgerbera 中。这些发现为苎麻属物种在传统医学中的正确鉴定和标准建立提供了初步的科学依据。
