Arias-Jayo Nerea, Alonso-Saez Laura, Ramirez-Garcia Andoni, Pardo Miguel A
1 Azti, Food Quality, Safety and Identity Department, Derio, Spain .
2 Azti, Marine Research Department. Sukarrieta, Spain .
Zebrafish. 2018 Apr;15(2):96-106. doi: 10.1089/zeb.2017.1460. Epub 2017 Dec 20.
The human intestine hosts a vast and complex microbial community that is vital for maintaining several functions related with host health. The processes that determine the gut microbiome composition are poorly understood, being the interaction between species, the external environment, and the relationship with the host the most feasible. Animal models offer the opportunity to understand the interactions between the host and the microbiota. There are different gnotobiotic mice or rat models colonized with the human microbiota, however, to our knowledge, there are no reports on the colonization of germ-free zebrafish with a complex human intestinal microbiota. In the present study, we have successfully colonized 5 days postfertilization germ-free zebrafish larvae with the human intestinal microbiota previously extracted from a donor and analyzed by high-throughput sequencing the composition of the transferred microbial communities that established inside the zebrafish gut. Thus, we describe for first time which human bacteria phylotypes are able to colonize the zebrafish digestive tract. Species with relevant interest because of their linkage to dysbiosis in different human diseases, such as Akkermansia muciniphila, Eubacterium rectale, Faecalibacterium prausnitzii, Prevotella spp., or Roseburia spp. have been successfully transferred inside the zebrafish digestive tract.
人类肠道中存在着庞大而复杂的微生物群落，这对于维持与宿主健康相关的多种功能至关重要。目前，人们对决定肠道微生物群组成的过程了解甚少，其中物种间相互作用、外部环境以及与宿主的关系是最可能的因素。动物模型为理解宿主与微生物群之间的相互作用提供了契机。有不同的定菌小鼠或大鼠模型被人类微生物群定植，然而，据我们所知，尚无关于无菌斑马鱼被复杂的人类肠道微生物群定植的报道。在本研究中，我们成功地用先前从供体中提取并通过高通量测序分析在斑马鱼肠道内定植的转移微生物群落组成的人类肠道微生物群，对受精后5天的无菌斑马鱼幼体进行了定植。因此，我们首次描述了哪些人类细菌系统型能够在斑马鱼消化道中定植。由于与不同人类疾病中的生态失调有关而具有相关研究意义的物种，如嗜黏蛋白阿克曼氏菌、直肠真杆菌、普拉梭菌、普雷沃氏菌属或罗斯氏菌属，已成功转移至斑马鱼消化道内。
