Endo T, Saito T, Onaya T
Third Department of Internal Medicine, University of Yamanashi Medical School, Japan.
J Histochem Cytochem. 1989 Apr;37(4):547-9. doi: 10.1177/37.4.2926129.
We introduce a new method for immunofluorescence detection of soluble material by blotting unfixed antigens onto nitrocellulose (NC) sheets. Human thyroid sections cut with a cryostat were mounted on NC sheets instead of glass slides and were air-dried. They were stained by immunofluorescence techniques, using autoantibodies to thyroid antigens obtained from 13 patients with chronic thyroiditis. The structures of the follicle lumen and epithelium were visualized by localization of specific antibodies. This tissue-blotting method sensitively detected soluble antigens, such as thyroglobulin, which are difficult to detect by conventional methods.
我们介绍了一种通过将未固定的抗原印迹到硝酸纤维素（NC）膜上来进行可溶性物质免疫荧光检测的新方法。用低温恒温器切割的人甲状腺切片被放置在NC膜上而非载玻片上，然后进行空气干燥。使用从13例慢性甲状腺炎患者获得的甲状腺抗原自身抗体，通过免疫荧光技术对其进行染色。通过特异性抗体的定位观察到了滤泡腔和上皮的结构。这种组织印迹方法能够灵敏地检测到诸如甲状腺球蛋白等传统方法难以检测的可溶性抗原。
