一种由人骨髓单核细胞系产生的新型单核细胞趋化和激活因子的纯化与特性分析
Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line.
作者信息
Matsushima K, Larsen C G, DuBois G C, Oppenheim J J
机构信息
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, MD 21701.
出版信息
J Exp Med. 1989 Apr 1;169(4):1485-90. doi: 10.1084/jem.169.4.1485.
Abstract
A novel basic heparin-binding monocyte chemotactic factor (MCF) was purified to homogeneity from the conditioned media of human myelomonocytic cell line THP-1 based on its in vitro monocyte chemotactic activity. The purified MCF was homogenous and estimated to be 15 kD on SDS-PAGE. Purified MCF stimulated normal human monocytes to be growth inhibitory in vitro at 2-3 d for several human tumor cell lines. This represents the first report of the identification and purification of a chemoattractant cytokine that also activates monocytes but is distinct from interferons and other known cytokines.
摘要
一种新型的碱性肝素结合单核细胞趋化因子（MCF），基于其体外单核细胞趋化活性，从人骨髓单核细胞系THP-1的条件培养基中纯化至同质。纯化后的MCF是同质的，在SDS-PAGE上估计为15kD。纯化后的MCF在体外2-3天可刺激正常人单核细胞对多种人类肿瘤细胞系产生生长抑制作用。这是关于一种趋化因子细胞因子的鉴定和纯化的首次报道，该细胞因子也能激活单核细胞，但不同于干扰素和其他已知的细胞因子。
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