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载铑柠檬酸根的磁赤铁矿纳米粒子对荷乳腺癌小鼠纤维连接蛋白和层粘连蛋白表达的调控作用。

Modulation of fibronectin and laminin expression by Rhodium (II) citrate-coated maghemite nanoparticles in mice bearing breast tumor.

机构信息

Nanoscience and Nanotechnology Postgraduate Program, University of Brasília, 70.910-900, Brasília, DF, Brazil.

Institute of Biological Sciences, University of Brasília, 70.910-900, Brasília, DF, Brazil.

出版信息

Sci Rep. 2017 Dec 20;7(1):17904. doi: 10.1038/s41598-017-18204-1.

DOI:10.1038/s41598-017-18204-1
PMID:29263369
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5738373/
Abstract

Degradation of cellular matrix is one of the important processes related to the progression of breast cancer. Tumor cells have the ability to exhibit necessary conditions for growth and survival, promoting degradation processes of extracellular matrix proteins, such as laminin (LN) and fibronectin (FN). In this study, we evaluated whether treatments, based on free rhodium (II) citrate (Rh(Hcit)), maghemite nanoparticles coated with citrate (Magh-cit) and maghemite nanoparticles coated with rhodium (II) citrate (Magh-Rh(Hcit)), in murine metastatic breast carcinoma models can modulate the expression of laminin and fibronectin proteins. Synthesized nanoparticles were characterized using X-ray diffraction, transmission electron microscopy, energy dispersive spectroscopy and dynamic light scattering. The expression of FN and LN was assessed using immunohistochemistry and western blotting. The gene expression of FN1 and LAMA1 were evaluated using real-time PCR. The FN1 and LAMA1 transcripts from the Magh-Rh(Hcit) treated group were 95% and 94%, respectively, lower than the control group. Significant reduction in tumor volume for animals treated with Magh-Rh(Hcit) was observed, of about 83%. We witnessed statistically significant reductions of FN and LN expression following treatment with Magh-Rh(Hcit). We have demonstrated that the antitumor effects of Magh-Rh(Hcit) and Rh(Hcit) regulate the expression of FN and LN in metastatic breast tumors.

摘要

细胞基质的降解是与乳腺癌进展相关的重要过程之一。肿瘤细胞具有表现出生长和存活所需条件的能力，促进细胞外基质蛋白如层粘连蛋白 (LN) 和纤维连接蛋白 (FN) 的降解过程。在这项研究中，我们评估了基于游离铑 (II) 柠檬酸盐 (Rh(Hcit))、柠檬酸包覆的磁赤铁矿纳米粒子 (Magh-cit) 和柠檬酸包覆的铑 (II) 柠檬酸盐 (Magh-Rh(Hcit)) 的治疗方法，能否调节在小鼠转移性乳腺癌模型中，层粘连蛋白和纤维连接蛋白蛋白的表达。使用 X 射线衍射、透射电子显微镜、能量色散光谱和动态光散射对合成的纳米粒子进行了表征。使用免疫组织化学和蛋白质印迹法评估 FN 和 LN 的表达。使用实时 PCR 评估 FN1 和 LAMA1 的基因表达。Magh-Rh(Hcit) 处理组的 FN1 和 LAMA1 转录本分别降低了 95%和 94%，低于对照组。用 Magh-Rh(Hcit) 处理的动物的肿瘤体积显著减小，约为 83%。我们观察到在用 Magh-Rh(Hcit) 治疗后 FN 和 LN 的表达有统计学意义的降低。我们已经证明，Magh-Rh(Hcit) 和 Rh(Hcit) 的抗肿瘤作用调节转移性乳腺癌中 FN 和 LN 的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/72ae61a6b9f0/41598_2017_18204_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/0e2b429c9faf/41598_2017_18204_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/be6b6c042ce7/41598_2017_18204_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/613d7a220e05/41598_2017_18204_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/cb4b4f309e94/41598_2017_18204_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/540cc98dfc79/41598_2017_18204_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/202d68bcf2ee/41598_2017_18204_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/72ae61a6b9f0/41598_2017_18204_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/0e2b429c9faf/41598_2017_18204_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/be6b6c042ce7/41598_2017_18204_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/613d7a220e05/41598_2017_18204_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/cb4b4f309e94/41598_2017_18204_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/540cc98dfc79/41598_2017_18204_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/202d68bcf2ee/41598_2017_18204_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbcb/5738373/72ae61a6b9f0/41598_2017_18204_Fig7_HTML.jpg

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