Chaves Natalia L, Estrela-Lopis Irina, Böttner Julia, Lopes Cláudio Ap, Guido Bruna C, de Sousa Aparecido R, Báo Sônia N
Institute of Biological Sciences, Department of Cell Biology, University of Brasília (UnB), Brasília, Brazil.
Institute of Biophysics and Medical Physics, University of Leipzig, Leipzig, Germany.
Int J Nanomedicine. 2017 Aug 2;12:5511-5523. doi: 10.2147/IJN.S141582. eCollection 2017.
Nanocarriers have the potential to improve the therapeutic index of currently available drugs by improving their efficacy and achieving therapeutic steady-state levels over an extended period. The association of maghemite-rhodium citrate (MRC) nanoparticles (NPs) has the potential to increase specificity of the cytotoxic action. However, the interaction of these NPs with cells, their uptake mechanism, and subcellular localization need to be elucidated. This work evaluates the uptake mechanism of MRC NPs in metastatic and nonmetastatic breast cancer-cell models, comparing them to a nontumor cell line. MRC NPs uptake in breast cancer cells was more effective than in normal cells, with regard to both the amount of internalized material and the achievement of more strategic intracellular distribution. Moreover, this process occurred through a clathrin-dependent endocytosis pathway with different basal expression levels of this protein in the cell lines tested.
纳米载体有潜力通过提高现有药物的疗效并在较长时间内达到治疗稳态水平来改善其治疗指数。柠檬酸磁赤铁矿 - 铑（MRC）纳米颗粒（NPs）的结合有可能增加细胞毒性作用的特异性。然而，这些纳米颗粒与细胞的相互作用、它们的摄取机制以及亚细胞定位需要阐明。这项工作评估了MRC纳米颗粒在转移性和非转移性乳腺癌细胞模型中的摄取机制，并将它们与非肿瘤细胞系进行比较。就内化物质的量和更具策略性的细胞内分布的实现而言，MRC纳米颗粒在乳腺癌细胞中的摄取比在正常细胞中更有效。此外，这个过程是通过网格蛋白依赖性内吞途径发生的，在测试的细胞系中这种蛋白具有不同的基础表达水平。
