Wanek N, Muneoka K, Holler-Dinsmore G, Burton R, Bryant S V
Developmental Biology Center, University of California, Irvine 92717.
J Exp Zool. 1989 Jan;249(1):41-9. doi: 10.1002/jez.1402490109.
A series of 15 stages of development for the mouse limb bud have been defined, spanning the time from the first appearance of the limb bud to the completion of limb outgrowth. The stages are based on changes in the morphology of the limb in living preparations. The development and regression of the apical ectodermal ridge (AER) as well as the development of the skeletal structures are also described. This staging system has been developed in response to the need to standardize in situ experimental analyses of the mouse limb bud. Comparable stages of the commonly used chick wing and mouse whole embryo systems are presented.
已经确定了小鼠肢芽发育的15个阶段，涵盖从肢芽首次出现到肢体生长完成的时间段。这些阶段基于活体标本中肢体形态的变化。还描述了顶端外胚层嵴（AER）的发育和退化以及骨骼结构的发育。这个分期系统是为满足对小鼠肢芽进行原位实验分析标准化的需求而开发的。文中还给出了常用的鸡翼和小鼠全胚胎系统的可比阶段。
