Liu Rongfu, Qin Xiangcheng, Ji Chengyong, Zeng Weixin, Yang Yufeng, Tan Wei
Department of Urology, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Department of Urology, Ningbo Yinzhou Second Hospital, Ningbo, China.
Asian J Urol. 2015 Jul;2(3):151-157. doi: 10.1016/j.ajur.2015.06.009. Epub 2015 Jul 18.
Human () was recently discovered to be a component of the Wnt signaling pathway required for β-catenin/Tcf-mediated transcription. But the role of in malignant cell proliferation and invasion has not yet been determined.
Lentivirus-mediated small interfering RNA (siRNA) and vector-based overexpression were used to study the function of in OS-RC-2 cells. The resulted cells were subject to Western blotting assay, MTT assay, colony formation and cell invasion assays. Furthermore, renal cell carcinoma (RCC) models were established in BALB/c nude mice inoculated with OS-RC-2 cells. Immunohistochemistry (IHC) staining of matrix metalloproteinase-7 (MMP-7), matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) was performed in tumor tissue.
gene was successful knocked down and overexpressed in RCC OS-RC-2 cells by using an shRNA and overexpressing vector, respectively. Overexpression of effectively promoted cell proliferation, colony formation and invasion . Knockdown of obviously inhibited xenograft tumor growth in nude mice. In addition, overexpression of increased the levels of MMP-7, MMP-9 and VEGF in the xenograft tumors.
has a role in promoting cell proliferation, invasion and metastasis, and may regulate angiogenesis via the Wnt/β-catenin signaling pathway.
人类[具体基因名称未给出]最近被发现是β-连环蛋白/Tcf介导转录所需的Wnt信号通路的一个组成部分。但[具体基因名称未给出]在恶性细胞增殖和侵袭中的作用尚未确定。
利用慢病毒介导的小干扰RNA（siRNA）和基于载体的过表达来研究[具体基因名称未给出]在OS-RC-2细胞中的功能。对所得细胞进行蛋白质免疫印迹分析、MTT分析、集落形成和细胞侵袭分析。此外，在接种OS-RC-2细胞的BALB/c裸鼠中建立肾细胞癌（RCC）模型。对肿瘤组织进行基质金属蛋白酶-7（MMP-7）、基质金属蛋白酶-9（MMP-9）和血管内皮生长因子（VEGF）的免疫组织化学（IHC）染色。
分别使用shRNA和过表达载体成功在肾癌细胞系OS-RC-2中敲低和过表达了[具体基因名称未给出]基因。[具体基因名称未给出]的过表达有效促进了细胞增殖、集落形成和侵袭。敲低[具体基因名称未给出]明显抑制了裸鼠体内异种移植肿瘤的生长。此外，[具体基因名称未给出]的过表达增加了异种移植肿瘤中MMP-7、MMP-9和VEGF的水平。
[具体基因名称未给出]在促进细胞增殖、侵袭和转移中起作用，并且可能通过Wnt/β-连环蛋白信号通路调节血管生成。
