Ahmadian Shalchi Fereshteh, Hosseini Nayyerehalsadat, Nourmohammadi Fatemeh, Ahmadian Shalchi Mohammad Arian, Forghanifard Mohammad Mahdi
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
BMC Mol Cell Biol. 2025 Apr 29;26(1):14. doi: 10.1186/s12860-025-00540-0.
Esophageal squamous cell carcinoma (ESCC), one of the most aggressive carcinomas of the gastrointestinal tract, is the sixth most common cause of cancer-related death. Wnt pathway plays a pivotal role in cell proliferation and differentiation. PYGO2 and IL10 are involved in this pathway. Our aim in this study was to examine the correlation between PYGO2 and IL10 expression in ESCC patients and cell lines.
Relative-comparative real time-PCR (RT-qPCR) was used to evaluate the PYGO2 and IL10 mRNA expression profile in 58 non-treated ESCC compared to their margin normal tissues. Expression of PYGO2 was induced in KYSE-30 and YM1 ESCC lines and IL10 expression was analyzed.
The results revealed the significant overexpression of PYGO2 and IL10 mRNA in 31.0% and 51.7% of ESCCs, respectively. The PYGO2 and IL10 overexpression was significantly correlated to each other (p = 0.007). Concomitant overexpression of the genes was significantly associated to grade of tumor differentiation (p < 0.01), and tumor depth of invasion (p < 0.05). Induced expression of PYGO2 caused a meaningful change in IL10 expression in ESCC cells.
PYGO2 may regulate IL10 through Wnt/β-catenin signaling pathway, suggesting a possible oncogenic role for PYGO2/IL10 axis in ESCC tumorigenesis. Considering the involvement of IL10 as an anti-inflammatory cytokine and PYGO2 role in elevated tumor invasion and metastasis, possible functional interaction between these factors may promote a process which induces invasion and malignant phenotype in ESCC.
食管鳞状细胞癌（ESCC）是胃肠道最具侵袭性的癌症之一，是癌症相关死亡的第六大常见原因。Wnt通路在细胞增殖和分化中起关键作用。PYGO2和IL10参与该通路。本研究的目的是检测ESCC患者及细胞系中PYGO2与IL10表达之间的相关性。
采用相对比较实时定量聚合酶链反应（RT-qPCR）评估58例未经治疗的ESCC与其癌旁正常组织中PYGO2和IL10 mRNA表达谱。在KYSE-30和YM1 ESCC细胞系中诱导PYGO2表达并分析IL10表达。
结果显示，分别有31.0%和51.7%的ESCC中PYGO2和IL10 mRNA显著过表达。PYGO2和IL10的过表达彼此显著相关（p = 0.007）。基因的同时过表达与肿瘤分化程度（p < 0.01）和肿瘤浸润深度（p < 0.05）显著相关。诱导PYGO2表达导致ESCC细胞中IL10表达发生有意义的变化。
PYGO2可能通过Wnt/β-连环蛋白信号通路调节IL10，提示PYGO2/IL10轴在ESCC肿瘤发生中可能具有致癌作用。鉴于IL10作为抗炎细胞因子的作用以及PYGO2在肿瘤侵袭和转移增加中的作用，这些因子之间可能的功能相互作用可能促进ESCC中诱导侵袭和恶性表型的过程。
