Majid Farjana, Jahan Munira, Lutful Moben Ahmed, Tabassum Shahina
Department of Microbiology, Tairunnessa Memorial Medical College, Tongi, Gazipur, Bangladesh.
Department of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh.
Euroasian J Hepatogastroenterol. 2014 Jan-Jun;4(1):31-35. doi: 10.5005/jp-journals-10018-1093. Epub 2014 Jan 22.
Both real-time-polymerase chain reaction (PCR) and hybrid capture 2 (HC2) assay can detect and quantify hepatitis B virus (HBV) DNA. However, real-time-PCR can detect a wide range of HBV DNA, while HC2 assay could not detect lower levels of viremia. The present study was designed to detect and quantify HBV DNA by real-time-PCR and HC2 assay and compare the quantitative data of these two assays.
A cross-sectional study was conducted in between July 2010 and June 2011. A total of 66 serologically diagnosed chronic hepatitis B (CHB) patients were selected for the study. Real-time-PCR and HC2 assay was done to detect HBV DNA. Data were analyzed by statistical Package for the social sciences (SPSS).
Among 66 serologically diagnosed chronic hepatitis B patients 40 (60.61%) patients had detectable and 26 (39.39%) had undetectable HBV DNA by HC2 assay. Concordant results were obtained for 40 (60.61%) out of these 66 patients by real-time-PCR and HC2 assay with mean viral load of 7.06 ± 1.13 log copies/ml and 6.95 ± 1.08 log copies/ml, respectively. In the remaining 26 patients, HBV DNA was detectable by real-time-PCR in 20 patients (mean HBV DNA level was 3.67 ± 0.72 log copies/ml. However, HBV DNA could not be detectable in six cases by the both assays. The study showed strong correlation (r = 0.915) between real-time-PCR and HC2 assay for the detection and quantification of HBV DNA.
HC2 assay may be used as an alternative to real-time-PCR for CHB patients. Majid F, Jahan M, Moben AL, Tabassum S. Comparison of Hybrid Capture 2 Assay with Real-time-PCR for Detection and Quantitation of Hepatitis B Virus DNA. Euroasian J Hepato-Gastroenterol 2014;4(1):31-35.
实时聚合酶链反应（PCR）和杂交捕获2（HC2）检测法均可检测和定量乙型肝炎病毒（HBV）DNA。然而，实时PCR能检测广泛范围的HBV DNA，而HC2检测法无法检测到较低水平的病毒血症。本研究旨在通过实时PCR和HC2检测法检测和定量HBV DNA，并比较这两种检测法的定量数据。
于2010年7月至2011年6月进行了一项横断面研究。共选择66例血清学诊断为慢性乙型肝炎（CHB）的患者进行研究。采用实时PCR和HC2检测法检测HBV DNA。数据通过社会科学统计软件包（SPSS）进行分析。
在66例血清学诊断为慢性乙型肝炎的患者中，HC2检测法检测到40例（60.61%）患者的HBV DNA可检测，26例（39.39%）患者的HBV DNA不可检测。这66例患者中，40例（60.61%）通过实时PCR和HC2检测法获得了一致结果，平均病毒载量分别为7.06±1.13 log拷贝/毫升和6.95±1.08 log拷贝/毫升。在其余26例患者中，实时PCR检测到20例患者的HBV DNA可检测（平均HBV DNA水平为3.67±0.72 log拷贝/毫升）。然而，两种检测法均有6例患者的HBV DNA不可检测。该研究表明，实时PCR和HC2检测法在检测和定量HBV DNA方面具有很强的相关性（r = 0.915）。
HC2检测法可作为CHB患者实时PCR的替代方法。马吉德F、贾汉M、莫本AL、塔巴苏姆S。杂交捕获2检测法与实时PCR检测乙型肝炎病毒DNA的比较。《欧亚肝脏胃肠病学杂志》2014年；4（1）：31 - 35。
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