Nakazato M, Tanaka M, Matsukura S, Kangawa K, Matsuo H
Third Department of Internal Medicine, Miyazaki Medical College, Japan.
J Neurol Sci. 1989 Feb;89(2-3):235-42. doi: 10.1016/0022-510x(89)90025-7.
The purpose of this study was to develop a quantitative method for variant transthyretin (also called prealbumin) in amyloid-laden tissues of familial amyloidotic polyneuropathy (FAP). The variant transthyretin was extracted by using 70% formic acid solution in which the protein was solubilized completely and rapidly. The quantity of the variant transthyretin was measured by a radioimmunoassay (RIA). An immunoreactive species detected in the tissues of FAP was confirmed to be the variant transthyretin by reverse-phase high-performance liquid chromatography. The quantity of the variant transthyretin in the thyroid gland of 2 FAP patients was 9.90 and 9.76 micrograms/mg tissue, and that of the kidney was 4.03 and 6.27 micrograms/mg tissue, respectively, while its quantity in the liver was only 0.15 and 0.12 microgram/mg tissue. We present here a quantitative analysis of the distribution and predominance of amyloid deposits in systemic organs of FAP.
本研究的目的是开发一种定量方法，用于检测家族性淀粉样多神经病（FAP）淀粉样变组织中的变异甲状腺素运载蛋白（也称为前白蛋白）。采用70%甲酸溶液提取变异甲状腺素运载蛋白，该溶液能使蛋白质迅速完全溶解。通过放射免疫分析（RIA）测定变异甲状腺素运载蛋白的含量。通过反相高效液相色谱法证实，在FAP组织中检测到的一种免疫反应性物质为变异甲状腺素运载蛋白。两名FAP患者甲状腺组织中变异甲状腺素运载蛋白的含量分别为9.90和9.76微克/毫克组织，肾脏中分别为4.03和6.27微克/毫克组织，而肝脏中仅为0.15和0.12微克/毫克组织。我们在此展示了FAP全身器官中淀粉样沉积物分布和优势的定量分析。
