School of Chemical Engineering, University of Ulsan, Ulsan, 680-749, Republic of Korea.
Department of Biotechnology, Indian Institute of Technology Madras, Chennai, Tamil nadu, 600036, India.
Bioprocess Biosyst Eng. 2018 Apr;41(4):479-487. doi: 10.1007/s00449-017-1882-z. Epub 2017 Dec 20.
Peptide-displaying Escherichia coli cells were investigated for use in adsorptive removal of bisphenol A (BPA) both in Luria-Bertani medium including BPA or ATM thermal paper eluted wastewater. Two recombinant strains were constructed with monomeric and dimeric repeats of the 7-mer BPA-binding peptide (KSLENSY), respectively. Greater than threefold increased adsorption of BPA [230.4 µmol BPA per g dry cell weight (DCW)] was found in dimeric peptide-displaying cells compared to monomeric strains (63.4 µmol per g DCW) in 15 ppm BPA solution. The selective removal of BPA from a mixture of BPA analogs (bisphenol F and bisphenol S) was verified in both monomeric and dimeric peptide-displaying cells. The binding chemistry of BPA with the peptide was assumed, based on molecular docking analysis, to be the interaction of BPA with serine and asparagine residues within the 7-mer peptide sequence. The peptide-displaying cells also functioned efficiently in thermal paper eluted wastewater containing 14.5 ppm BPA.
展示肽的大肠杆菌细胞被研究用于吸附去除双酚 A（BPA），包括在含有 BPA 的 Luria-Bertani 培养基中或 ATM 热敏纸洗脱废水中。分别构建了带有单体和二聚体重复的 7 肽 BPA 结合肽（KSLENSY）的两种重组菌株。在 15ppm BPA 溶液中，与单体菌株（每克干细胞重量 63.4µmol）相比，二聚体肽展示细胞对 BPA 的吸附增加了三倍以上[每克 DCW 230.4µmol BPA]。在单体和二聚体肽展示细胞中都验证了从 BPA 类似物（双酚 F 和双酚 S）混合物中选择性去除 BPA。基于分子对接分析，假设 BPA 与肽的结合化学是 BPA 与 7 肽序列内丝氨酸和天冬酰胺残基的相互作用。肽展示细胞在含有 14.5ppm BPA 的热敏纸洗脱废水中也能有效发挥作用。
