Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Org Biomol Chem. 2018 Jan 17;16(3):372-375. doi: 10.1039/c7ob02871g.
This work studies strand displacement in the context of DNA triplexes. We observed that toehold-mediated strand displacement could proceed in DNA triplexes, but at a much slower rate than in DNA duplexes. In this study, strand displacement was studied with native polyacrylamide gel electrophoresis (PAGE) for two molecular systems. While both systems were involved in branch migration in a DNA triplex, the toehold forms either a duplex or a triplex. This study provides a new strategy for sequence-specific strand displacement and it is useful for the construction of dynamic DNA nanostructures and control of smart DNA nanodevices.
这项工作研究了 DNA 三链体结构中链置换的情况。我们观察到，在 DNA 三链体中可以进行引发链介导的链置换，但速度比 DNA 双链体慢得多。在这项研究中，使用 native polyacrylamide gel electrophoresis (PAGE) 对两个分子系统进行了链置换研究。虽然这两个系统都参与了 DNA 三链体中的分支迁移，但引发链形成双链体或三链体。本研究为序列特异性链置换提供了一种新策略，对于构建动态 DNA 纳米结构和控制智能 DNA 纳米器件具有重要意义。
