Department of Chemistry, New York University, New York, New York 10003, United States.
The RNA Institute, University of Albany, State University of New York, Albany, New York 12222, United States.
J Am Chem Soc. 2023 Feb 15;145(6):3599-3605. doi: 10.1021/jacs.2c12667. Epub 2023 Feb 2.
Reconfigurable structures engineered through DNA hybridization and self-assembly offer both structural and dynamic applications in nanotechnology. Here, we have demonstrated that strand displacement of triplex-forming oligonucleotides (TFOs) can be translated to a robust macroscopic DNA crystal by coloring the crystals with covalently attached fluorescent dyes. We show that three different types of triplex strand displacement are feasible within the DNA crystals and the bound TFOs can be removed and/or replaced by (a) changing the pH from 5 to 7, (b) the addition of the Watson-Crick complement to a TFO containing a short toehold, and (c) the addition of a longer TFO that uses the duplex edge as a toehold. We have also proved by X-ray diffraction that the structure of the crystals remains as designed in the presence of the TFOs.
通过 DNA 杂交和自组装设计的可重构结构在纳米技术中具有结构和动态应用。在这里，我们通过将共价连接的荧光染料染色晶体，证明三链体形成寡核苷酸 (TFO) 的链置换可以转化为坚固的宏观 DNA 晶体。我们表明，在 DNA 晶体中可以实现三种不同类型的三链体链置换，并且可以通过以下方式去除和/或替换结合的 TFO：(a) 将 pH 值从 5 变为 7，(b) 将 Watson-Crick 互补物添加到含有短结合点的 TFO 中，以及 (c) 添加使用双链边缘作为结合点的更长 TFO。我们还通过 X 射线衍射证明，在存在 TFO 的情况下，晶体的结构仍然保持设计。
