依赖 NADPH 的 5-酮-D-葡萄糖酸还原酶是真菌 D-葡萄糖醛酸分解代谢途径的一部分。
NADPH-dependent 5-keto-D-gluconate reductase is a part of the fungal pathway for D-glucuronate catabolism.
机构信息
VTT Technical Research Centre of Finland Ltd, Espoo, Finland.
出版信息
FEBS Lett. 2018 Jan;592(1):71-77. doi: 10.1002/1873-3468.12946. Epub 2017 Dec 30.
NADPH-dependent 5-keto-D-gluconate reductase was identified as a missing element in the pathway for D-glucuronate catabolism in fungi. The disruption of the gene, gluF, by CRISPR/Cas9 in the filamentous fungus Aspergillus niger resulted in a strain unable to catabolise D-glucuronate. The purified GluF protein was characterized and k and K values of 23.7 ± 1.8 s and 3.2 ± 0.1 mm for 5-keto-D-gluconate, respectively, were determined. The enzyme is reversible and is active with NADP and D-gluconate. We suggest a pathway for D-glucuronate catabolism with the intermediates L-gulonate, 2-keto-L-gulonate, L-idonate, 5-keto-D-gluconate, D-gluconate and D-gluconate-6-phosphate which is a part of the pentose phosphate pathway. A fungal enzyme activity for the conversion of L-gulonate to 2-keto-L-gulonate remains to be identified.
NADPH 依赖的 5-酮-D-葡萄糖酸还原酶被鉴定为真菌中 D-葡萄糖酸分解途径中的一个缺失元素。通过 CRISPR/Cas9 在丝状真菌黑曲霉中对基因 gluF 的破坏，导致菌株无法分解 D-葡萄糖酸。纯化的 GluF 蛋白被表征，确定了分别为 5-酮-D-葡萄糖酸的 k 和 K 值为 23.7±1.8 s 和 3.2±0.1 mm。该酶是可逆的，可与 NADP 和 D-葡萄糖酸一起作用。我们提出了 D-葡萄糖酸分解代谢的途径，其中包括中间体 L-古洛糖酸、2-酮-L-古洛糖酸、L-异头酸、5-酮-D-葡萄糖酸、D-葡萄糖酸和 D-葡萄糖酸-6-磷酸，这些都是戊糖磷酸途径的一部分。真菌中 L-古洛糖酸转化为 2-酮-L-古洛糖酸的酶活性仍有待确定。
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