拟南芥花粉伸展蛋白 LRX 在花粉管生长过程中对于细胞壁的完整性是必需的。
Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth.
机构信息
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, "Dr. Héctor Torres" (INGEBI-CONICET), Buenos Aires, Argentina.
Fundación Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina.
出版信息
FEBS Lett. 2018 Jan;592(2):233-243. doi: 10.1002/1873-3468.12947. Epub 2018 Jan 5.
Proper cell wall assembly is crucial during pollen tube growth. Leucine-rich repeat extensins (LRXs) are extracellular glycoproteins which belong to the hydroxyproline-rich glycoprotein (HRGP) family. They contain a conserved N-terminal leucine-rich repeat (LRR) domain and a highly variable C-terminal extensin domain. Here, we characterized four LRX proteins (LRX8 through LRX11) from pollen of Arabidopsis thaliana. To investigate the role of LRX8-LRX11 in pollen germination and pollen tube growth, multiple T-DNA lrx mutants were obtained. The lrx mutants display abnormal pollen tubes with an irregular deposition of callose and pectin. They also show serious alterations in pollen germination and segregation ratio. Our results suggest that LRXs are involved in ensuring proper cell wall assembly during pollen tube growth.
正确的细胞壁组装在花粉管生长过程中至关重要。富含亮氨酸的重复扩展蛋白（LRXs）是属于富含羟脯氨酸的糖蛋白（HRGP）家族的细胞外糖蛋白。它们含有保守的 N 端亮氨酸丰富重复（LRR）结构域和高度可变的 C 端扩展蛋白结构域。在这里，我们从拟南芥花粉中鉴定了四个 LRX 蛋白（LRX8 到 LRX11）。为了研究 LRX8-LRX11 在花粉萌发和花粉管生长中的作用，获得了多个 T-DNA lrx 突变体。这些 lrx 突变体显示出花粉管异常，具有不规则的胼胝质和果胶沉积。它们在花粉萌发和分离比上也表现出严重的改变。我们的结果表明，LRXs 参与确保花粉管生长过程中正确的细胞壁组装。
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