Department of Plastic & Cosmetic Surgery, Maternal and Child Health Medical Institute, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China.
J Cell Biochem. 2018 Jun;119(6):4636-4643. doi: 10.1002/jcb.26632. Epub 2018 Feb 28.
Endogenous peptides play crucial roles in various biological processes. Their effects on the pathogenesis of human infantile hemangioma remains poorly understood. In this study, we construct a comparative peptidomic profiling between human infantile hemangioma tissue and matched normal skin using liquid chromatography-tandem mass spectrometry. A total of 192 peptides were significantly differentially expressed in human infantile hemangioma tissue, with 182 upregulated, and 10 downregulated peptides between infantile hemangioma tissue and matched normal skin. Performing bioinformatics analysis (Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis), we found that precursor proteins of these differentially expressed peptides correlate with metabolic process, biological regulation, binding, catalytic activity, and pathways in cancer occurring during pathological changes of infantile hemangioma. Furthermore, 89 differentially expressed peptides shared homology with antimicrobial peptides and 13 matched known immunomodulatory peptides based on prediction database. In conclusion, our results reveal significantly altered expression profiles of peptides in human infantile hemangioma tissue. These peptides may participate in the etiology of infantile hemangioma and provide beneficial scheme for clinical treatments.
内源性肽在各种生物过程中发挥着关键作用。它们对人类婴儿血管瘤发病机制的影响仍知之甚少。在这项研究中，我们使用液相色谱-串联质谱法在人类婴儿血管瘤组织和匹配的正常皮肤之间构建了比较肽组图谱。在人类婴儿血管瘤组织中，共有 192 种肽显著差异表达，其中 182 种上调，10 种下调。对这些差异表达肽的前体蛋白进行生物信息学分析（基因本体论和京都基因与基因组百科全书通路分析），发现它们与代谢过程、生物调节、结合、催化活性以及婴儿血管瘤病理变化过程中的癌症通路相关。此外，根据预测数据库，89 种差异表达肽与抗菌肽同源，13 种与已知的免疫调节肽匹配。总之，我们的研究结果揭示了人类婴儿血管瘤组织中肽的表达谱发生了显著改变。这些肽可能参与了婴儿血管瘤的病因，并为临床治疗提供了有益的方案。
