Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital, Obsterics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China.
Department of Neonatology, Wuxi Children's Hospital affiliated to Nanjing Medical University, Wuxi, Jiangsu.
J Cell Biochem. 2018 Feb;119(2):1866-1878. doi: 10.1002/jcb.26347. Epub 2017 Sep 7.
To gain insight into the effect of metformin on losing weight from peptidomic perspective and to screen potential active peptides for reducing fat lipid deposition. After determining the proper concentration of metformin on human primary visceral adipocytes, we constructed a comparative peptidomic profiling between control and metformin treatment group (n = 3) using a stable isobaric labeling strategy involving tandem mass tag reagents, followed by liquid chromatography tandem mass spectrometry. We identified and quantified 3065 non-redundant peptides, 304 of which were differentially expressed after metformin treatment, 206 peptides were up regulated and 98 peptides were down regulated significantly. Gene ontology (GO) enrichment and pathway analysis were performed to study differentially peptides though their precursor proteins. We concluded three peptides located within the functional domains of their precursor proteins could be candidate bioactive peptides for obesity. On one hand, these results confirmed the versatile effects of metformin on adipocyte and advance our current understanding of metformin, on the other hand, these identified peptides might play putative roles in treatment of obesity.
为了从肽组学角度深入了解二甲双胍减肥的效果，并筛选出潜在的活性肽来减少脂肪脂质沉积。在确定了二甲双胍对人原代内脏脂肪细胞的适当浓度后，我们使用涉及串联质量标签试剂的稳定同位素标记策略，在对照组和二甲双胍治疗组（n=3）之间构建了比较肽组学分析，随后进行液相色谱串联质谱分析。我们鉴定和定量了 3065 个非冗余肽，其中 304 个在二甲双胍处理后表达差异，206 个肽上调，98 个肽下调显著。通过其前体蛋白进行基因本体（GO）富集和途径分析，研究差异肽。我们得出结论，三个位于其前体蛋白功能域内的肽可能是肥胖的候选生物活性肽。一方面，这些结果证实了二甲双胍对脂肪细胞的多效作用，加深了我们对二甲双胍的现有认识，另一方面，这些鉴定出的肽可能在肥胖症的治疗中发挥作用。
