Bafort Françoise, Damblon Christian, Smargiasso Nicolas, De Pauw Edwin, Perraudin Jean-Paul, Jijakli Mohamed Haïssam
Integrated and Urban Plant Pathology Laboratory, Gembloux Agro-Bio Tech, Liège University, Passage des Déportés 2, 5030, Gembloux, Belgium.
Structural Biological Chemistry Laboratory (SBCL), Liège University, 4000, Liège, Belgium.
Chem Biodivers. 2018 Mar;15(3):e1700497. doi: 10.1002/cbdv.201700497. Epub 2018 Mar 7.
The potential of ions produced in water by the lactoperoxidase system against plant pests has shown promising results. We tested the bioactivity of ions produced by the lactoperoxidase oxidation of I and SCN in several buffers or in tap water and characterized the ions produced. In vitro biological activity was tested against Penicillium expansum, the causal agent of mold in fruits, and the major cause of patulin contamination of fruit juices and compotes. In buffers, the ionic concentration was increased 3-fold, and pathogen inhibition was obtained down to the 1:15 dilution. In tap water, the ionic concentration was weaker, and pathogen inhibition was obtained only down to the 1:3 dilution. Acidic buffer increased ion concentrations as compared to less acidic (pH 5.6 or 6.2) or neutral buffers, as do increased ionic strength. C-labelled SCN and MS showed that different ions were produced in water and in buffers. In specific conditions the ion solution turned yellow and a product was formed, probably diiodothiocyanate (I SCN ), giving an intense signal at 49.7 ppm in C-NMR. The formation of the signal was unambiguously favored in acidic media and disadvantaged or inhibited in neutral or basic conditions. It was enhanced at a specific SCN : I ratio of 1:4.5, but decreased when the ratio was 1:2, and was inhibited at ratio SCN >I . We demonstrated that the formation of the signal required the interaction between I and SCN , and MS showed the presence of I SCN .
