肺结核患者中基因的甲基化状态。
The methylation state of gene in pulmonary tuberculosis patients.
作者信息
Jiang Chao, Zhu Junjie, Liu Yuxiao, Luan Xidi, Jiang Yan, Jiang Gening, Fan Jiang
机构信息
Department of Thoracic Surgery, Shanghai Pulmonary Hospital Affiliated Tongji University, Shanghai 200433, China.
Shandong Energy Zibo Mining Group Co., LTD Central Hospital, Zibo 255120, China.
出版信息
J Thorac Dis. 2017 Nov;9(11):4353-4357. doi: 10.21037/jtd.2017.09.107.
BACKGROUND
Our previous study suggested that the methylation of vitamin D receptor () gene affected its expression and the defense against tuberculosis (TB) infection . In this study, we further investigated the methylation level of gene in pulmonary TB patients.
METHODS
The consecutive TB patients who were admitted into our department from January 2013 to January 2015 were recruited. The potential methylation sites analyzed in this study included 16 CpG sites. The amplification of bisulfite modified genomic DNA was ligated to pUC18-T vector and the positive clone of blue-white selection was sequenced to analyze the methylation state of gene.
RESULTS
This study had samples of 27 TB patients and 30 healthy controls. TB patients were in the hyper-methylation state compared to the healthy controls. The significant differences between TB patients and healthy controls were detected in 7 of these 16 CpG sites. The mRNA expression of AKT, GSK3βand FOXO1 decreased in TB patients compared to that of healthy controls.
CONCLUSIONS
Our study contributes to supplying more evidences that the methylation level of gene affects the progression of TB.
背景
我们之前的研究表明，维生素D受体（）基因的甲基化会影响其表达以及对结核病（TB）感染的防御能力。在本研究中，我们进一步调查了肺结核患者中基因的甲基化水平。
方法
招募了2013年1月至2015年1月期间连续入住我科的肺结核患者。本研究分析的潜在甲基化位点包括16个CpG位点。对亚硫酸氢盐修饰的基因组DNA进行扩增，连接到pUC18 - T载体上，并对蓝白筛选的阳性克隆进行测序，以分析基因的甲基化状态。
结果
本研究有27例肺结核患者和30例健康对照的样本。与健康对照相比，肺结核患者处于高甲基化状态。在这16个CpG位点中的7个位点检测到肺结核患者与健康对照之间存在显著差异。与健康对照相比，肺结核患者中AKT、GSK3β和FOXO1的mRNA表达降低。
结论
我们的研究有助于提供更多证据表明基因的甲基化水平会影响结核病的进展。
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