State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, China.
University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
Anal Chem. 2018 Feb 6;90(3):2141-2147. doi: 10.1021/acs.analchem.7b04428. Epub 2018 Jan 12.
In the present work, an enhanced and stable anodic electrochemiluminescence (ECL) was observed from a suspension of boron nitride quantum dots (BNQDs) and Ru(bpy), which had a 400-fold enhancement compared with individual Ru(bpy). Interestingly, different from the previous research on BNQDs as a type of optical probe, BNQDs were demonstrated as an efficient coreactant of Ru(bpy)-based ECL for the first time and confirmed by collecting the ECL spectra. The amino-bearing groups and the electrocatalytic effect of the BNQDs endowed them as potential coreactants for ECL of Ru(bpy), and the possible mechanism of the electrode surface reaction was discussed. Several factors including electrode material, the pH of the buffer solution, and the amount of BNQDs were investigated and also further confirmed the role of the BNQDs in the proposed Ru(bpy)/BNQDs system. On the basis of the quenching effect between the excited state of Ru(bpy) and the oxidation form of DA in the ECL system of Ru(bpy)/BNQDs, the ECL sensing platform for DA was successfully established. The proposed ECL system with the outstanding ECL efficiency may hold great potential in the bioanlysis because of the biocompatibility and good stability of BNQDs.
在本工作中，观察到了来自氮化硼量子点（BNQDs）和 Ru(bpy) 的悬浮液中的增强和稳定的阳极电致化学发光（ECL），与单独的 Ru(bpy) 相比，其增强了 400 倍。有趣的是，与之前将 BNQDs 作为一种光学探针的研究不同，BNQDs 首次被证明为基于 Ru(bpy) 的 ECL 的有效共反应物，并通过收集 ECL 光谱进行了确认。BNQDs 所具有的含氨基基团和电催化效应使它们成为 Ru(bpy) ECL 的潜在共反应物，并且讨论了电极表面反应的可能机制。研究了包括电极材料、缓冲溶液的 pH 值和 BNQDs 的量在内的多个因素，并且还进一步证实了 BNQDs 在所提出的 Ru(bpy)/BNQDs 体系中的作用。基于 Ru(bpy)/BNQDs ECL 体系中 Ru(bpy) 的激发态与 DA 的氧化形式之间的猝灭效应，成功建立了用于 DA 的 ECL 传感平台。由于 BNQDs 的生物相容性和良好的稳定性，具有出色 ECL 效率的所提出的 ECL 系统可能在生物分析中具有很大的潜力。
