Wen Qi-Tao, Wang Tao, Yu Da-Hai, Wang Zheng-Rui, Sun Ying, Liang Cui-Wei
Dental Medical Center, People's Hospital of Hainan Province, Haikou 570100, China.
Dental Medical Center, People's Hospital of Hainan Province, Haikou 570100, China.
Asian Pac J Trop Med. 2017 Dec;10(12):1177-1184. doi: 10.1016/j.apjtm.2017.10.026. Epub 2017 Oct 29.
To develop a BALB/c mouse model of oral submucous fibrosis (OSF) induced by arecoline and to exhibit an accumulation of collagen and angiogenesis changes.
BALB/c mice were randomly assigned to either the control (distilled water) or experimental group (arecoline) (n = 40). Eight mice from each group were sacrificed every 4 weeks since 8 weeks post treatment. Changes in histopathologic features, levels of collagen type I and collagen type III, and angiogenesis were measured.
In the 8th week, epithelium atrophy, collagen cumulation and micrangium pathologic changes in the lamina propria were observed in the oral mucosa. In the 20th week, hyaline degeneration of the connective tissues was observed on the tongue and palate mucosa. The angiogenesis and collagen type I changed significantly as the diseases advanced (P < 0.05); however, collagen type III was not statistically different.
An OSF model involving mice can be rapidly induced by drinking a high-dose of arecoline. OSF angiogenic changes in mice primarily decrease and collagen accumulation is mainly collagen type I.
建立槟榔碱诱导的BALB/c小鼠口腔黏膜下纤维化（OSF）模型，并展示胶原蛋白的积累和血管生成变化。
将BALB/c小鼠随机分为对照组（蒸馏水）和实验组（槟榔碱）（n = 40）。自治疗后8周起，每组每4周处死8只小鼠。测量组织病理学特征、I型和III型胶原蛋白水平以及血管生成的变化。
第8周时，在口腔黏膜中观察到上皮萎缩、固有层胶原蛋白累积和微血管病理变化。第20周时，在舌和腭黏膜上观察到结缔组织的透明变性。随着疾病进展，血管生成和I型胶原蛋白发生显著变化（P < 0.05）；然而，III型胶原蛋白无统计学差异。
通过饮用高剂量槟榔碱可快速诱导小鼠OSF模型。小鼠OSF血管生成变化主要减少，胶原蛋白积累主要为I型胶原蛋白。
