从贝宁土壤中分离得到的作为异小杆线虫和斯氏线虫共生菌的发光杆菌属和嗜线虫致病杆菌属细菌的分子多样性。
Molecular diversity of Photorhabdus and Xenorhabdus bacteria, symbionts of Heterorhabditis and Steinernema nematodes retrieved from soil in Benin.
作者信息
Godjo Anique, Afouda Leonard, Baimey Hugues, Decraemer Wilfrida, Willems Anne
机构信息
Department of Crop Production, Faculty of Agronomy, University of Parakou, 02 B.P. 1003, Parakou, Benin.
Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, 9000, Ghent, Belgium.
出版信息
Arch Microbiol. 2018 May;200(4):589-601. doi: 10.1007/s00203-017-1470-2. Epub 2017 Dec 21.
The diversity of 43 bacterial strains isolated from Beninese entomopathogenic nematodes was investigated molecularly by analyzing the 16S rRNA, recA, and gyrB genes. Based on 16S rRNA sequence analysis, 15 bacterial strains were identified as Xenorhabdus sp., 27 strains as Photorhabdus sp., and one as Serratia sp. The Xenorhabdus strains were isolated from Steinernema nematodes and identified as Xenorhabdus indica based on 16S rRNA gene and concatenated recA and gyrB sequence analysis. However, analysis of 16S rRNA and concatenated recA and gyrB gene sequences of the Photorhabdus strains, all isolated from Heterorhabditis nematodes, resulted in two separate sub-clusters (A) and (B) within the Photorhabdus luminescens group, distinct from the existing subspecies. They share low sequence similarities with nearest phylogenetic neighbors Photorhabdus luminescens subsp. luminescens Hb, Photorhabdus luminescens subsp. caribbeanensis HG29, and Photorhabdus luminescens subsp. noenieputensis AM7.
通过分析16S rRNA、recA和gyrB基因，对从贝宁昆虫病原线虫中分离出的43株细菌菌株的多样性进行了分子研究。基于16S rRNA序列分析，15株细菌菌株被鉴定为嗜线虫致病杆菌属（Xenorhabdus sp.），27株为发光杆菌属（Photorhabdus sp.），1株为沙雷氏菌属（Serratia sp.）。嗜线虫致病杆菌属菌株从斯氏线虫属（Steinernema）线虫中分离得到，并基于16S rRNA基因以及recA和gyrB序列的串联分析鉴定为印度嗜线虫致病杆菌（Xenorhabdus indica）。然而，对所有从异小杆线虫属（Heterorhabditis）线虫中分离得到的发光杆菌属菌株的16S rRNA以及recA和gyrB基因序列的串联分析，在发光杆菌（Photorhabdus luminescens）组内产生了两个独立的亚群（A）和（B），与现有的亚种不同。它们与最近的系统发育邻居发光杆菌发光亚种（Photorhabdus luminescens subsp. luminescens）Hb、发光杆菌加勒比海亚种（Photorhabdus luminescens subsp. caribbeanensis）HG29和发光杆菌诺尼普滕西斯亚种（Photorhabdus luminescens subsp. noenieputensis）AM7的序列相似性较低。
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