Zhu Ming-Lin, Zhao Jin-Ping, Cui Ning, Gonçalves-Rizzi Victor H, Possomato-Vieira Jose S, Nascimento Regina A, Dias-Junior Carlos A
Department of Thoracic and Cardiovascular Surgery, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
J Huazhong Univ Sci Technolog Med Sci. 2017 Dec;37(6):904-909. doi: 10.1007/s11596-017-1825-6. Epub 2017 Dec 21.
Myeloperoxidase (MPO) is released from activated neutrophils. The inflammation in preeclampsia was found to be associated with endothelial dysfunction. We hypothesized that cardiac and circulating MPO levels are elevated in hypertensive pregnancy. Systolic and diastolic blood pressure and heart rate were measured on pregnancy days 14, 16, 18 and 20 in normal pregnant and hypertensive pregnant rats. Left and right ventricle weights, the number of viable fetuses, litter size, fetal and placenta weights were recorded on gestational day 21. Circulating and cardiac MPO activities, soluble fms-like tyrosine kinase-1 (sFlt-1) and vascular endothelial growth factor (VEGF) and nitric oxide (NO) were detected. The results showed increases in cardiac (left, but not right ventricle) and circulating MPO activities, and concomitantly lower number of viable fetuses, litter size, and fetal and placenta weights, and decreases in NO in hypertensive pregnant rats. Also, the increases in circulating sFlt-1 and VEGF were found in hypertensive pregnant group. In conclusion, maternal and fetal detrimental changes along with increases in circulating sFlt-1 and VEGF in hypertensive pregnancy may be associated with increases in cardiac and circulating MPO activities, confirming the causative role of inflammatory response in preeclampsia.
髓过氧化物酶（MPO）由活化的中性粒细胞释放。子痫前期的炎症被发现与内皮功能障碍有关。我们假设高血压妊娠时心脏和循环中的MPO水平会升高。在正常妊娠和高血压妊娠大鼠的妊娠第14、16、18和20天测量收缩压、舒张压和心率。在妊娠第21天记录左右心室重量、存活胎儿数量、窝仔数、胎儿和胎盘重量。检测循环和心脏中的MPO活性、可溶性fms样酪氨酸激酶-1（sFlt-1）、血管内皮生长因子（VEGF）和一氧化氮（NO）。结果显示，高血压妊娠大鼠心脏（左心室，但右心室未出现）和循环中的MPO活性增加，同时存活胎儿数量、窝仔数以及胎儿和胎盘重量降低，NO减少。此外，高血压妊娠组循环中的sFlt-1和VEGF增加。总之，高血压妊娠时母体和胎儿的有害变化以及循环中sFlt-1和VEGF的增加可能与心脏和循环中MPO活性的增加有关，证实了炎症反应在子痫前期中的致病作用。
