Clowes A W, Clowes M M, Fingerle J, Reidy M A
Department of Surgery, University of Washington School of Medicine, Seattle.
Lab Invest. 1989 Mar;60(3):360-4.
Arteries denuded by the passage of a balloon catheter demonstrate marked smooth muscle cell (SMC) proliferation, whereas arteries denuded by a fine wire do not. We examined the possibility that vessel distension in addition to endothelial loss accounted for the SMC proliferation in the balloon model. Segments of rat left carotid isolated by temporary ligatures were distended hydrostatically with tissue culture medium at 300 mm Hg pressure via an external carotid cannula. Animals received either continuous [3H]thymidine infusion (intraperitoneally, Alzet pump, Alz Corp., Palo Alza, California) for 14 days or pulsed with [3H]thymidine at 1, 2, 3, or 7 days, and SMC proliferation was assessed by autoradiography. Distended vessels demonstrated patchy denudation acutely and complete endothelial regeneration by 3 days. No SMC were present in the intima at later times. Nevertheless, cumulative SMC proliferation over 14 days was 21 +/- 3% (perfused but not distended control: 0.8 +/- 0.6%; partially de-endothelialized but not distended control: 0.3 +/- 0.1%). Pulse-labeling demonstrated a peak of SMC proliferation between 2 and 3 days after injury. These results suggest that acute distension can contribute to the induction of SMC proliferation after endothelial denudation.
被球囊导管通过的动脉显示出明显的平滑肌细胞（SMC）增殖，而被细钢丝通过的动脉则没有。我们研究了除内皮细胞丢失外，血管扩张是否是球囊模型中SMC增殖的原因。通过临时结扎分离的大鼠左颈动脉段，通过颈外动脉插管在300 mmHg压力下用组织培养基进行液压扩张。动物接受连续14天的[3H]胸腺嘧啶核苷输注（腹腔内，Alzet泵，Alz公司，加利福尼亚州帕洛阿尔托）或在第1、2、3或7天用[3H]胸腺嘧啶核苷脉冲给药，并通过放射自显影评估SMC增殖。扩张的血管急性显示出斑片状剥脱，3天时内皮完全再生。后期内膜中不存在SMC。然而，14天内累积的SMC增殖为21±3%（灌注但未扩张的对照：0.8±0.6%；部分去内皮但未扩张的对照：0.3±0.1%）。脉冲标记显示损伤后2至3天SMC增殖达到峰值。这些结果表明，急性扩张可在内皮剥脱后促进SMC增殖。
