Guzmán David Calderón, Herrera Maribel Ortiz, Brizuela Norma Osnaya, Mejía Gerardo Barragán, García Ernestina Hernández, Olguín Hugo Juárez, Ruíz Norma Labra, Peraza Armando Valenzuela
Laboratorio de Neurociencias, Instituto Nacional de Pediatría (INP), Mexico, Mexico.
Laboratorio de Bacteriología Experimental, INP, SSA, Mexico.
APMIS. 2018 Feb;126(2):128-134. doi: 10.1111/apm.12794. Epub 2017 Dec 21.
The aim of this study was to determine the effect of oseltamivir and indomethacin on lipid peroxidation (LP), GABA levels, and ATPase activity in brain and stomach of normal and infected rats (IR), as novel inflammation model. Female Sprague Dawley rats grouped five each, either in the absence or presence of a live culture of Salmonella typhimurium (S. typh), were treated as follows: group 1 (control), PBS buffer; group 2, oseltamivir (100 mg/kg); group 3, indomethacin (67 μg/rat); group 4, oseltamivir (100 mg/kg) + indomethacin (67 μg/rat). All drugs were given intraperitoneally for 5 days. IR received the same treatments and the brain and stomach of the rats were removed in order to measure levels of GABA, LP, and total ATPase, using validated methods. Levels of GABA increased in stomach and cortex of IR with oseltamivir, but decreased in striatum and cerebellum/medulla oblongata of IR with indomethacin. LP decreased in the three brain regions of IR with oseltamivir. ATPase increased in stomach of IR and non-IR with oseltamivir and in striatum and cerebellum/medulla oblongata of IR with indomethacin. Results suggest that the effect of free radicals produced in an infection and inflammatory condition caused by S. typh could be less toxic by a combination of oseltamivir and indomethacin.
