Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), México.
Andrologia. 2011 Aug;43(4):266-72. doi: 10.1111/j.1439-0272.2010.01067.x. Epub 2011 Feb 25.
The aim of this study was to evaluate the effect of sildenafil and prostaglandin E1 (PGE1) (drugs used in erectile dysfunction) on production of free radicals in prostate and brain of rat. A single dose of sildenafil (10 mg kg(-1) ) and PGE1 (20 μg kg(-1) ) was given to Sprague-Dawley rats (300 g weight) intraperitoneally. The levels of testosterone were measured in blood. Their brains and prostate glands were separated to measure lipid peroxidation, Na(+) and K(+) ATPase activity, reduced glutathione (GSH) and serotonin levels, by means of validated methods. The levels of testosterone increased slightly in animals treated with sildenafil and PGE1. The activity of total ATPase was increased in the prostate of animals treated with sildenafil + PGE1 but decreased in those that received sildenafil alone. PGE1 caused significant diminution of GSH levels in both organs. Sildenafil increased the levels of serotonine in brain, whereas in prostate they decreased instead. Our results suggest that sildenafil induced changes in GSH levels as well as in the serotonergic metabolism, alone or with PGE1 in prostate and brain, respectively. Thus, the combination therapy may be ideal to sustain the biochemical balance due to biphasic stimulation on brain and prostate.
本研究旨在评估西地那非和前列腺素 E1（PGE1）（用于治疗勃起功能障碍的药物）对大鼠前列腺和大脑中自由基生成的影响。将单剂量的西地那非（10mg/kg）和 PGE1（20μg/kg）给腹腔注射到 Sprague-Dawley 大鼠（体重 300g）中。通过验证方法测量血液中的睾酮水平。分离其大脑和前列腺，以测量脂质过氧化、Na(+)和 K(+)ATP 酶活性、还原型谷胱甘肽（GSH）和 5-羟色胺水平。用西地那非和 PGE1 处理的动物的睾酮水平略有升高。与单独使用西地那非的动物相比，用西地那非+PGE1 处理的动物的总 ATP 酶活性在前列腺中增加。PGE1 导致两种器官的 GSH 水平显著降低。西地那非增加了大脑中的 5-羟色胺水平，而在前列腺中则降低了。我们的结果表明，西地那非单独或与 PGE1 一起分别在前列腺和大脑中引起 GSH 水平以及 5-羟色胺代谢的变化。因此，联合治疗可能是维持生化平衡的理想选择，因为对大脑和前列腺有双相刺激作用。
