Department of Joint and Sports Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
Department of Rehabilitation, East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
Chin Med J (Engl). 2018 Jan 5;131(1):69-74. doi: 10.4103/0366-6999.221264.
Adipose-derived stromal vascular fraction (ADSVF) can be applied to repair tendon and ligament tears. ADSVF treatment has a better therapeutic potential than adipose stem cells alone in promoting the healing of connective tissue injury in rabbit models. Magnetic resonance imaging (MRI) and biomechanical testing were used in this study to evaluate the efficiency of SVF in the healing of tendon-bone interface of a rotator cuff injury after reattachment.
A total of 36 rabbits were studied between March and June 2016, 18 rabbits received the SVF-fibrin glue (SVF-FG) treatment and the other 18 formed the control group. ADSVF was isolated from each rabbit. A bilateral amputation of the supraspinatus tendon and parallel reconstruction was also performed on all the 36 rabbits. Then, a mixture of SVF and FG was injected into the tendon-bone interface of the SVF-FG group, whereas the control group only received FG. The animals were randomly sacrificed at 4, 8, and 12 weeks after surgery (n = 6 per group), respectively. The shoulders were prepared for MRI scanning and analysis of biomechanical properties. Analyses of variance were performed using SPSS 13.0.
MRI scanning showed that the signal-to-noise quotient of the SVF-FG group was not significantly higher than that of the control group at either 4 (20.1 ± 3.6 vs. 18.2 ± 3.4, F = 1.570, P = 0.232) or 8 weeks (20.7 ± 3.3 vs. 18.0 ± 3.0, F = 2.162, P = 0.117) posttreatment, and only became significant after 12 weeks (27.5 ± 4.6 vs. 22.1 ± 1.9, F = 4.968, P = 0.009). Biomechanical properties such as the maximum load, maximum strength, and the stiffness for the SVF-FG group were significantly greater than that for the control group at 8 weeks' posttreatment (maximum load: 166.89 ± 11.62 N vs. 99.40 ± 5.70 N, P < 0.001; maximum strength: 8.22 ± 1.90 N/mm vs. 5.82 ±0.68 N/mm, P < 0.010; and the stiffness: 34.85± 3.00 Pa vs. 24.57± 5.72 Pa, P < 0.010).
Local application of ADSVF might lead to better tendon-bone healing in rabbit models.
脂肪来源的基质血管部分（ADSVF）可用于修复肌腱和韧带撕裂。在促进兔模型中结缔组织损伤愈合方面，ADSVF 治疗比单独的脂肪干细胞具有更好的治疗潜力。本研究采用磁共振成像（MRI）和生物力学测试来评估 SVF 在附着后修复肩袖损伤的腱骨界面中的效率。
2016 年 3 月至 6 月，共研究了 36 只兔子，18 只兔子接受了 SVF-纤维蛋白胶（SVF-FG）治疗，另 18 只兔子形成对照组。从每只兔子中分离 ADSVF。所有 36 只兔子均进行双侧肩袖肌腱切断术和并行重建。然后，将 SVF 和 FG 的混合物注入 SVF-FG 组的腱骨界面，而对照组仅接受 FG。手术后分别在 4、8 和 12 周（每组 6 只）随机处死动物。对肩部进行 MRI 扫描和生物力学性能分析。采用 SPSS 13.0 进行方差分析。
MRI 扫描显示，SVF-FG 组的信噪比在治疗后 4 周（20.1 ± 3.6 对 18.2 ± 3.4，F = 1.570，P = 0.232）或 8 周（20.7 ± 3.3 对 18.0 ± 3.0，F = 2.162，P = 0.117）时均未显著高于对照组，仅在 12 周后才变得显著（27.5 ± 4.6 对 22.1 ± 1.9，F = 4.968，P = 0.009）。SVF-FG 组的最大载荷、最大强度和刚度等生物力学性能在治疗后 8 周时明显大于对照组（最大载荷：166.89 ± 11.62 N 对 99.40 ± 5.70 N，P < 0.001；最大强度：8.22 ± 1.90 N/mm 对 5.82 ± 0.68 N/mm，P < 0.010；和刚度：34.85 ± 3.00 Pa 对 24.57 ± 5.72 Pa，P < 0.010）。
局部应用 ADSVF 可能会导致兔模型中的腱骨愈合更好。
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