采用超高效液相色谱-质谱联用技术分析生地黄和盐制地黄的化学成分变化。
Analysis of Chemical Variations between Crude and Salt-Processed Anemarrhenae rhizoma Using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry Methods.
机构信息
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
出版信息
Molecules. 2017 Dec 22;23(1):23. doi: 10.3390/molecules23010023.
The present study was designed to systematically investigate the chemical profile differences between crude (CAR) and salt-processed (SAR). Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS), coupled with multivariate statistical analysis was used for the discrimination of chemical profiles and the identification of the differentiation of the chemical constitutions of CAR and SAR. In addition, seven main constituents of CAR and SAR were simultaneously determined by ultra-high-performance liquid chromatography-quadrupole mass spectrometry (UHPLC-MS) for analyzing the content variations. A total of 24 components were found to be the main contributors to the significant difference between CAR and SAR. The structures of the marker compounds were identified based on their chromatographic behaviors, intact precursor ions, and characteristic MS fragmentation patterns. The potential structural transformation mechanism of furostanol saponins during salt processing was explored. The results may provide a scientific foundation for deeply elucidating the processing mechanism of .
本研究旨在系统地研究生地黄（CAR）和盐制地黄（SAR）之间的化学成分差异。采用超高效液相色谱-四极杆飞行时间质谱联用技术（UHPLC-QTOF-MS），结合多元统计分析，对CAR 和 SAR 的化学成分进行区分和鉴定。此外，还采用超高效液相色谱-四极杆质谱联用技术（UHPLC-MS）同时测定 CAR 和 SAR 的七种主要成分，分析其含量变化。结果表明，24 种成分是造成 CAR 和 SAR 显著差异的主要原因。根据其色谱行为、完整的前体离子和特征 MS 裂解模式，鉴定了标记化合物的结构。探讨了呋甾烷醇皂苷在盐加工过程中的潜在结构转化机制。研究结果为深入阐明地黄的炮制机制提供了科学依据。
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