Department of Head and Neck Surgery, Jiangxi Cancer Hospital, Nanchang City, Jiangxi Province, Nanchang, China.
Eur Rev Med Pharmacol Sci. 2017 Dec;21(24):5638-5647. doi: 10.26355/eurrev_201712_14007.
MicroRNAs (miRs) are critical regulators in cancer development and progression. The current study aimed to investigate the expression and potential function of miR-181a in thyroid cancer.
A total of 15 paired thyroid cancer tissues and adjacent normal tissues were subjected to Real-time Polymerase Chain Reaction (PCR) to evaluate miR-181a expression. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, enzyme linked immunosorbent assay (ELISA) or flow cytometry was employed to assess the growth activity, apoptosis and cell cycle, respectively, upon modulation of the miR-181a expression in TPC-1 cells. Western blot was used to assess protein expression. The interaction between miR-181a and RB1 was tested by luciferase activity assay.
The expression of miR-181a was significantly upregulated in thyroid cancer tissues compared with the adjacent tissues. Inhibition of miR-181a attenuated cell growth, which could be abrogated by miR-181a co-transfection. MiR-181a overexpression reduced apoptosis and promoted cell cycle progression; inhibition of miR-181a exerted opposite effects on both cell cycle and apoptosis. MiR-181a directly suppressed RB1 expression. RB1 expression in tumor tissues was downregulated and negatively correlated with miR-181a expression.
miR-181a plays an oncogenic role in thyroid cancer; by targeting RB1, it promotes cell cycle progression and inhibits apoptosis.
微小 RNA（miRs）是癌症发生和发展的关键调节因子。本研究旨在探讨 miR-181a 在甲状腺癌中的表达及潜在功能。
采用实时聚合酶链反应（PCR）检测 15 对甲状腺癌组织及相邻正常组织中 miR-181a 的表达。通过 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四唑溴盐（MTT）分析、酶联免疫吸附测定（ELISA）或流式细胞术分别评估 miR-181a 表达调控后 TPC-1 细胞的生长活性、凋亡和细胞周期。Western blot 用于评估蛋白表达。通过荧光素酶活性测定检测 miR-181a 与 RB1 之间的相互作用。
与相邻组织相比，甲状腺癌组织中 miR-181a 的表达明显上调。抑制 miR-181a 可减弱细胞生长，而 miR-181a 共转染可阻断其作用。miR-181a 过表达可减少细胞凋亡并促进细胞周期进程；抑制 miR-181a 则对细胞周期和凋亡产生相反的影响。miR-181a 可直接抑制 RB1 的表达。肿瘤组织中 RB1 的表达下调且与 miR-181a 的表达呈负相关。
miR-181a 在甲状腺癌中发挥致癌作用，通过靶向 RB1 促进细胞周期进程并抑制细胞凋亡。
