a Department of Biochemical Diagnostics , Medical University of Bialystok , Bialystok , Poland.
b Department of Pediatric Laboratory Diagnostics , Medical University of Bialystok , Bialystok , Poland.
Scand J Clin Lab Invest. 2018 Feb-Apr;78(1-2):125-130. doi: 10.1080/00365513.2017.1420217. Epub 2017 Dec 22.
The aim of this study was to evaluate the concentration of interleukin-6 and N-terminal propeptide of procollagen type I and their relationship in liver diseases of different etiologies.
Serum samples were obtained from 30 healthy volunteers and patients suffering from alcoholic cirrhosis (AC) - 31, non-alcoholic cirrhosis (NAC) - 28 and toxic hepatitis (HT) - 23 patients. Cirrhotic patients were classified according to Child-Pugh score. IL-6 and PINP concentrations were determined according to the electrochemiluminescence immunoassay.
The mean serum IL-6 concentration was significantly higher in AC (mean ± SD:21.52 ± 15.01 pg/mL), NAC (20.07 ± 32.12 pg/mL) and HT (15.14 ± 17.18 pg/mL) when compared to the control group (C) (1.67 ± 0.42 pg/mL) (Mann-Whitney U test: p < .001 for all comparisons). The mean serum PINP concentration was significantly higher only in patients with AC (104.32 ± 54.50 ng/mL) in comparison with the control group (54.70 ± 19.83 ng/mL; p < .001). The mean values of IL-6 and PINP significantly differed between liver diseases (ANOVA rank Kruskal-Wallis test: p = .020 and p < .001, respectively). Accordingly, the serum levels of IL-6 and PINP were significantly higher in patients with AC than that in NAC (p < .001 and p = .022, respectively). IL-6 and PINP concentrations appeared to vary depending on the severity of liver damage (p < .001 for both). The concentrations of IL-6 and PINP were significantly higher in class C (31.88 ± 21.51 pg/mL; 132.73 ± 65.63 ng/mL, respectively) than that in class A (6.12 ± 9.00 pg/mL; 57.32 ± 28.85 ng/mL, respectively) (p < .001 for both). There were also significant differences in IL-6 concentrations between Child-Pugh class B (27.88 ± 24.45 pg/mL) and class A (6.12 ± 9.00 pg/mL; p < .001).
We conclude that serum concentrations of IL-6 and PINP change in liver diseases, and those changes reflect the severity of liver disease.
本研究旨在评估不同病因肝病患者白细胞介素 6（IL-6）和 I 型前胶原氨基端肽（PINP）的浓度及其相关性。
采集 30 名健康志愿者和 31 名酒精性肝硬化（AC）、28 名非酒精性肝硬化（NAC）和 23 名中毒性肝炎（HT）患者的血清样本。根据 Child-Pugh 评分对肝硬化患者进行分类。采用电化学发光免疫分析法测定 IL-6 和 PINP 浓度。
与对照组（C）（1.67±0.42pg/mL）相比，AC（均值±标准差：21.52±15.01pg/mL）、NAC（20.07±32.12pg/mL）和 HT（15.14±17.18pg/mL）患者的血清 IL-6 浓度均显著升高（Mann-Whitney U 检验：所有比较均 p<0.001）。仅在 AC 患者中，血清 PINP 浓度（104.32±54.50ng/mL）明显高于对照组（54.70±19.83ng/mL；p<0.001）。IL-6 和 PINP 的均值在不同肝病间存在显著差异（方差分析秩 Kruskal-Wallis 检验：p=0.020 和 p<0.001）。因此，AC 患者的血清 IL-6 和 PINP 水平明显高于 NAC（p<0.001 和 p=0.022）。IL-6 和 PINP 浓度似乎随肝损伤严重程度而变化（均 p<0.001）。与 C 级（31.88±21.51pg/mL；132.73±65.63ng/mL）相比，A级（6.12±9.00pg/mL；57.32±28.85ng/mL）患者的 IL-6 和 PINP 浓度显著升高（均 p<0.001）。B 级（27.88±24.45pg/mL）和 A 级（6.12±9.00pg/mL）患者的 IL-6 浓度也存在显著差异（p<0.001）。
我们的结论是，血清中 IL-6 和 PINP 浓度在肝病中发生变化，这些变化反映了肝病的严重程度。
