Miura Tomoya, Matsubara Atsushi, Kudo Naomi, Hara Ryutaro, Takahata Junko, Sasaki Akira
a Department of Otorhinolaryngology , Hirosaki University Graduate School of Medicine , Hirosaki , Japan.
Acta Otolaryngol. 2018 May;138(5):447-451. doi: 10.1080/00016489.2017.1416170. Epub 2017 Dec 22.
In the present study, we aimed to clarify the expression of thymic stromal lymphopoietin (TSLP), a key trigger of Th2-type allergic disease, in the middle ear mucosa of eosinophilic otitis media (EOM).
An immunohistological study of TSLP was conducted in patients with EOM and in animal models of EOM constructed by intraperitoneal and intratympanic injection of ovalbumin for 7 and 14 days. In addition, the messenger RNA (mRNA) expression of TSLP in the middle ear mucosa of the animal models was analyzed using real-time PCR, and was compared with that of the control animals.
Immunoreactivities for TSLP were observed in the middle ear mucosa around the tympanic ostium of the eustachian tube of patients with EOM. In the animal model, strong immunoreactivity for TSLP was also observed in the eustachian tube epithelium, and mRNA expression of TSLP in the seven-day stimulated animals was significantly higher than that in the controls.
The present study indicates that the presence of epithelium-derived TSLP in the eustachian tubes plays an important role in the onset of EOM.
在本研究中，我们旨在阐明胸腺基质淋巴细胞生成素（TSLP），一种2型过敏性疾病的关键触发因子，在嗜酸性粒细胞性中耳炎（EOM）中耳黏膜中的表达情况。
对EOM患者以及通过腹腔和鼓室内注射卵清蛋白7天和14天构建的EOM动物模型进行TSLP的免疫组织学研究。此外，使用实时PCR分析动物模型中耳黏膜中TSLP的信使核糖核酸（mRNA）表达，并与对照动物进行比较。
在EOM患者咽鼓管鼓口周围的中耳黏膜中观察到TSLP的免疫反应性。在动物模型中，在咽鼓管上皮中也观察到TSLP的强免疫反应性，并且在刺激7天的动物中TSLP的mRNA表达明显高于对照动物。
本研究表明，咽鼓管中上皮源性TSLP的存在在EOM的发病中起重要作用。
