Pavey Warren, Raisis Anthea, Dunne Ben, Van Laeken Els, Jenkinson Charles, Vincent Viji, Baird Peter, Prince Stuart, Ho Kwok M, Merry Christopher, Gilfillan Ian
1 Fiona Stanley Hospital, Murdoch, Western Australia, Australia.
2 Murdoch University School of Veterinary and Life Sciences, Murdoch, Western Australia, Australia.
Perfusion. 2018 Jul;33(5):363-366. doi: 10.1177/0267659117746232. Epub 2017 Dec 22.
The isolated heart apparatus is over 100 years old, but remains a useful research tool today. While designs of many large animal systems have been described in the literature, trouble-shooting and refining such a model to yield a stable, workable system has not been previously described. This paper outlines the issues, in tabular form, that our group encountered in developing our own porcine isolated heart rig with the aim of assisting other workers in the field planning similar work. The paper also highlights some of the modern applications of the isolated heart apparatus. Methods Landrace pigs (50-80 kg) were used in a pilot project to develop the model. The model was then used in a study examining the effects of various cardioplegic solutions on function after reanimation of porcine hearts. During the two projects, non-protocol issues were documented as well as their solutions. These were aggregated in this paper.
Issues faced by the group without explicit literature solutions included pig size selection, animal acclimatisation, porcine transoesophageal echocardiography, cannulation and phlebotomy for cross-clamping, cardioplegia delivery, heart suspension and rig tuning.
Prior recognition of issues and possible solutions faced by workers establishing a porcine isolated heart system will speed progress towards a useable system for research. The isolated heart apparatus remains applicable in transplant, ischaemia reperfusion, heart failure and organ preservation research.
离体心脏装置已有100多年历史，但至今仍是一种有用的研究工具。虽然文献中已描述了许多大型动物系统的设计，但此前尚未描述过对这样一个模型进行故障排除和优化以获得稳定、可行系统的方法。本文以表格形式概述了我们团队在开发自己的猪离体心脏装置时遇到的问题，旨在帮助该领域计划开展类似工作的其他研究人员。本文还强调了离体心脏装置的一些现代应用。方法在一个试点项目中使用长白猪（50 - 80千克）来开发该模型。然后将该模型用于一项研究，考察各种心脏停搏液对猪心脏复苏后功能的影响。在这两个项目过程中，记录了非方案性问题及其解决方案，并汇总于此。
该团队面临的、无明确文献解决方案的问题包括猪的大小选择、动物适应、猪经食管超声心动图检查、交叉钳夹时的插管和放血、心脏停搏液的输送、心脏悬吊和装置调试。
预先认识到建立猪离体心脏系统的研究人员所面临的问题及可能的解决方案，将加快朝着获得可用研究系统的进展。离体心脏装置在移植、缺血再灌注、心力衰竭和器官保存研究中仍然适用。
