Portnoĭ V F, Machulin A V, Dvortsin G F
Kardiologiia. 1982 Jan;22(1):94-7.
The authors have elaborated and studied in 52 experiments an improved method of donor perfusion of an isolated dog heart. The technique of removing the heart without ischaemia and after cardioplegia as well as the technique of perfusion of the arrested and the contracting heart are described. The authors used their own method of the functional load testing of the left ventricle after cardioplegia. The function of the isolated dog heart without ischaemia was studied in control experiments. The advantages of the described method of perfusion and functional load of an isolated heart are: it is manageable, physiological and easily reproduced. The method can be used in experimental cardiology.
作者在52项实验中详细阐述并研究了一种改良的离体犬心供体灌注方法。描述了无缺血及心脏停搏后取出心脏的技术，以及灌注停搏心脏和跳动心脏的技术。作者采用了自己的心脏停搏后左心室功能负荷测试方法。在对照实验中研究了无缺血的离体犬心功能。所描述的离体心脏灌注和功能负荷方法的优点是：易于操作、符合生理且易于重复。该方法可用于实验心脏病学。
