Mohammadi-Ghalehbin Behnam, Hatam Gholamreza, Sarkari Bahador, Mohebali Mehdi, Zarei Zabih, Bohlooli Shahab
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Immunol. 2017 Dec;14(4):293-305.
Canine visceral leishmaniasis (CVL) caused by Leishmania infantum is endemic in the northwest and south of Iran. An appropriate vaccine can help to prevent and control visceral leishmaniasis in both humans and animals. Few studies have confirmed that the fucose-mannose ligand (FML) antigen of Leishmania donovani produced protective immunity in dogs against CVL.
To evaluate the immune responses of vaccinated dogs against FML antigen of L. infantum.
We isolated the FML antigen from native L. infantum and vaccinated the dogs with FML-saponin in an endemic area of VL in Iran to evaluate the immune responses of vaccinated dogs against this antigen.
Our results indicated a significant increase in the expression of IFN-γ, IL-10 and IL-13, but not IL-12A, gene transcripts in PBMCs of FML-saponin vaccinated dogs in comparison with controls. Our findings showed a significant difference in the ratio of IFN-γ/IL-10 mRNA expression in FML-saponin vaccinated dogs in comparison with two control groups. Moreover, a significant level of anti-FML antibodies was detected in serum of vaccinated dogs.
These findings showed that FML-saponin stimulates both Th1 and Th2 immune responses with predominant Th1 and strong humoral immune responses to produce protective immunity against CVL.
由婴儿利什曼原虫引起的犬内脏利什曼病（CVL）在伊朗西北部和南部流行。一种合适的疫苗有助于预防和控制人和动物的内脏利什曼病。很少有研究证实杜氏利什曼原虫的岩藻糖 - 甘露糖配体（FML）抗原能在犬中产生针对CVL的保护性免疫。
评估接种疫苗的犬对婴儿利什曼原虫FML抗原的免疫反应。
我们从本地婴儿利什曼原虫中分离出FML抗原，并在伊朗VL流行地区用FML - 皂苷给犬接种疫苗，以评估接种疫苗的犬对该抗原的免疫反应。
我们的结果表明，与对照组相比，FML - 皂苷接种犬的外周血单核细胞（PBMCs）中IFN - γ、IL - 10和IL - 13基因转录本的表达显著增加，但IL - 12A没有。我们发现FML - 皂苷接种犬的IFN - γ/IL - 10 mRNA表达比值与两个对照组相比有显著差异。此外，在接种疫苗犬的血清中检测到显著水平的抗FML抗体。
这些发现表明，FML - 皂苷刺激Th1和Th2免疫反应，以Th1为主导，并产生强烈的体液免疫反应，从而产生针对CVL的保护性免疫。
