Department of Materials Science and Engineering, Yonsei University , Seoul 03722, Korea.
Department of Biomedical Engineering, Gachon University , Incheon 21936, Korea.
ACS Sens. 2018 Jan 26;3(1):106-112. doi: 10.1021/acssensors.7b00681. Epub 2018 Jan 11.
In this work, the chronoamperometry-based redox cycling of 3,3',5,5'-tetramethylbenzidine (TMB) was performed by using interdigitated electrode (IDE). The signal was obtained from two sequential chronoamperometric profiles: (1) with the generator at the oxidative potential of TMB and the collector at the reductive potential of TMB, and (2) with the generator at the reductive potential of TMB and the collector at the oxidative potential of TMB. The chronoamperometry-based redox cycling (dual mode) showed a sensitivity of 1.49 μA/OD, and the redox cycling efficiency was estimated to be 94% (n = 10). The sensitivities of conventional redox cycling with the same interdigitated electrode and chronoamperometry using a single working electrode (single mode) were estimated to be 0.67 μA/OD and 0.18 μA/OD, respectively. These results showed that the chronoamperometry-based redox cycling (dual mode) could be more effectively used to quantify the oxidized TMB than other amperometric methods. The chronoamperometry-based redox cycling (dual mode) was applied to immunoassays using a commercial ELISA kit for medical diagnosis of the human hepatitis B virus surface antigen (hHBsAg). Finally, the chronoamperometry-based redox cycling (dual mode) provided more than a 10-fold higher sensitivity than conventional chronoamperometry using a single working electrode (single mode) when applied to a commercial ELISA kit for medical diagnosis of hHBsAg.
在这项工作中，通过使用叉指电极（IDE）进行了 3,3',5,5'-四甲基联苯胺（TMB）的计时安培氧化还原循环。该信号来自两个连续的计时安培曲线：（1）发生器处于 TMB 的氧化电势，收集器处于 TMB 的还原电势；（2）发生器处于 TMB 的还原电势，收集器处于 TMB 的氧化电势。基于计时安培的氧化还原循环（双模式）的灵敏度为 1.49 μA/OD，并且氧化还原循环效率估计为 94%（n = 10）。使用相同的叉指电极的传统氧化还原循环和使用单个工作电极的计时安培（单模式）的灵敏度分别估计为 0.67 μA/OD 和 0.18 μA/OD。这些结果表明，基于计时安培的氧化还原循环（双模式）可以比其他电流测定法更有效地用于定量氧化的 TMB。基于计时安培的氧化还原循环（双模式）应用于使用商业 ELISA 试剂盒进行的乙型肝炎病毒表面抗原（hHBsAg）的医学诊断的免疫测定。最后，基于计时安培的氧化还原循环（双模式）在应用于 hHBsAg 的商业 ELISA 试剂盒时，比使用单个工作电极的传统计时安培（单模式）提供了高 10 倍以上的灵敏度。
