Division of Bioengineering, College of Life Science and Bioengineering, Incheon National University, Republic of Korea; National Repository for Microalgae and Cyanobacteria - Freshwater (DBT, Govt. of India), School of Life Sciences, Bharathidasan University, Tiruchirappalli, India; Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India; Centre for Surface Technology and Applications, Korea Aerospace University, Republic of Korea.
Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India.
Microb Pathog. 2018 Feb;115:233-238. doi: 10.1016/j.micpath.2017.12.049. Epub 2017 Dec 22.
Fatty acid methyl esters (FAME) derived from lipids of microalgae is known to have wide bio-functional materials including antimicrobials. FAME is an ideal super-curator and superior anti-pathogenic. The present study evaluated the efficiency of FAME extracted from microalgae Scenedesmus intermedius as an antimicrobial agent against Gram positive (Staphylococcus aureus, Streptococcus mutans, and Bacillus cereus) Gram negative (Escherichia coli and Pseudomonas aeruginosa) bacteria and Fungi (Aspergillus parasiticus and Candida albicans). The minimal inhibitory concentration (MIC) for the gram negative bacteria was determined as 12-24 μg mL, whereas MIC for gram positive bacteria was 24-48 μg mL. MIC for the fungi was as high as 60-192 μg mL. The FAME profiles determined by gas chromatography showed 18 methyl esters. Among them, pharmacologically active FAME such as palmitic acid methyl ester (C16:0) was detected at high percentage (23.08%), which accounted for the bioactivity. FAME obtained in this study exhibited a strong antimicrobial activity at the lowest MIC than those of recent reports. This result clearly indicated that FAME of S. intermedius has a strong antimicrobial and antioxidant property and that could be used as an effective resource against microbial diseases.
脂肪酸甲酯（FAME）来源于微藻油脂，具有广泛的生物功能材料，包括抗菌剂。FAME 是一种理想的超级保护剂和优良的抗病原剂。本研究评估了从中间微藻 Scenedesmus intermedius 中提取的 FAME 作为抗菌剂对抗革兰氏阳性（金黄色葡萄球菌、变形链球菌和蜡状芽孢杆菌）、革兰氏阴性（大肠杆菌和铜绿假单胞菌）细菌和真菌（寄生曲霉和白色念珠菌）的效率。革兰氏阴性菌的最小抑菌浓度（MIC）为 12-24μg/mL，而革兰氏阳性菌的 MIC 为 24-48μg/mL。真菌的 MIC 高达 60-192μg/mL。气相色谱法测定的 FAME 图谱显示有 18 种甲酯。其中，高百分比（23.08%）检测到具有药理活性的 FAME，如棕榈酸甲酯（C16:0），这与其生物活性有关。本研究获得的 FAME 在最低 MIC 下表现出比最近报道更强的抗菌活性。这一结果清楚地表明，中间微藻的 FAME 具有很强的抗菌和抗氧化特性，可作为对抗微生物疾病的有效资源。
