Alcici Muhammed Abdelhameed Ismael, Abdelhaleem Salma Waheed, Fahim Karima Mogahed, Saleh Neveen Mohamed, Farag Heba Saeed
Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Department of Clinical Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
BMC Microbiol. 2025 Jan 29;25(1):54. doi: 10.1186/s12866-025-03747-5.
The excessive use of antibiotics is a major contributor to the global issue of antimicrobial resistance (AMR), a significant threat to human and animal health. Hence, assessing new strategies for managing Multi-Drug Resistant (MDR) microorganisms is vital. In this study, the use of mechanically isolated mature adipose cells (MIMACs) and their lysate (Adipolysate) as a new sustainable antimicrobial agent was assessed against Methicillin-resistant Staphylococcus aureus (MRSA).
The minimum volume of MIMACs achieved complete bacterial inhibition (Minimum Lethal volume) was 75 µl and 100 µl for bacterial concentration of 10 and 10 cfu/ml, respectively. Direct bacterial membrane attachment and intracellular capture was visualized under light and electron microscopy. Adipolysate was characterized via GC-MS, the fatty acid profile demonstrated several components with known antimicrobial properties. The tested Adipolysate revealed inhibition zone of diameter 25.33 ± 0.88 mm against the tested S. aureus strain, compared with the inhibition zone of Vancomycin (24.0 ± 0.00 mm) and Erythromycin (30.0 ± 0.00). The study revealed the potential effects of MIMACs and Adipolysate as sustainable, natural, and robust antimicrobial agents. However, these preliminary results will be further investigated to understand the mechanism of action and explore possible applications in various fields.
抗生素的过度使用是全球抗菌药物耐药性（AMR）问题的主要促成因素，对人类和动物健康构成重大威胁。因此，评估管理多重耐药（MDR）微生物的新策略至关重要。在本研究中，评估了使用机械分离的成熟脂肪细胞（MIMACs）及其裂解物（脂肪裂解物）作为一种新型可持续抗菌剂对耐甲氧西林金黄色葡萄球菌（MRSA）的作用。
对于细菌浓度为10⁶和10⁸ cfu/ml的情况，实现完全细菌抑制（最小致死体积）所需的MIMACs最小体积分别为75 μl和100 μl。在光学显微镜和电子显微镜下观察到细菌膜直接附着和细胞内捕获现象。通过气相色谱 - 质谱联用（GC-MS）对脂肪裂解物进行了表征，脂肪酸谱显示出几种具有已知抗菌特性的成分。与万古霉素（24.0±0.00 mm）和红霉素（30.0±0.00 mm）的抑菌圈相比，测试的脂肪裂解物对测试的金黄色葡萄球菌菌株显示出直径为25.33±0.88 mm的抑菌圈。该研究揭示了MIMACs和脂肪裂解物作为可持续、天然且强大的抗菌剂的潜在作用。然而，这些初步结果将进一步研究以了解其作用机制并探索在各个领域的可能应用。
