Posa Andreas, Paulsen Friedrich, Dietz Richard, Garreis Fabian, Sander Ralph, Schicht Martin, Sel Saadettin, Scholz Michael, Hammer Christian M, Bräuer Lars
Department of Neurology, Martin-Luther-University Halle-Wittenberg, Ernst-Grube-Straße 40, 06120 Halle (Saale), Germany.
Department of Anatomy II, Friedrich-Alexander-University Erlangen-Nürnberg, Universitätsstraße 19, 91054 Erlangen, Germany.
Ann Anat. 2018 Mar;216:90-94. doi: 10.1016/j.aanat.2017.11.006. Epub 2017 Dec 24.
To quantify and compare the amounts of surfactant proteins SP-A, SP-B, SP-C and SP-D in the tear fluid collected from patients with dry eye syndrome and from individuals with a healthy ocular surface.
Schirmer strips were used to collect tear fluid from both eyes of 241 volunteers (99 men, 142 women; age range: 18-87 years). Dry eye syndrome was diagnosed by ophthalmologists in 125 patients, whereas the healthy control group comprised 116 individuals. The total protein concentration was determined via Bradford assay. The relative concentration of surfactant proteins SP-A through -D was measured by enzyme-linked immuno-sorbent assay (ELISA).
The mean relative concentrations of SP-A, SP-C and SP-D were significantly higher in the dry eye group as compared to the healthy controls (p<0.05, one-way ANOVA). SP-B was also detected at a higher concentration in the dry eye group, but the difference to the control group was not statistically significant.
The upregulation of SP-A and SP-D in the dry eye group is probably related to these proteins' known antimicrobial and immunomodulatory effects at the ocular surface. It may represent a pathophysiological response to the inflammatory condition of the ocular surface in dry eye. The upregulation of SP-B and SP-C may represent an effort of the lacrimal system to reduce surface tension and thus to counteract the increased tendency of the tear film to tear in dry eye.
量化并比较从干眼症患者和眼表健康个体收集的泪液中表面活性蛋白SP-A、SP-B、SP-C和SP-D的含量。
使用Schirmer试纸从241名志愿者（99名男性，142名女性；年龄范围：18 - 87岁）的双眼收集泪液。125例患者经眼科医生诊断为干眼症，而健康对照组有116人。通过Bradford法测定总蛋白浓度。采用酶联免疫吸附测定（ELISA）法测量表面活性蛋白SP-A至SP-D的相对浓度。
与健康对照组相比，干眼症组中SP-A、SP-C和SP-D的平均相对浓度显著更高（p<0.05，单因素方差分析）。干眼症组中SP-B的浓度也较高，但与对照组的差异无统计学意义。
干眼症组中SP-A和SP-D的上调可能与这些蛋白在眼表已知的抗菌和免疫调节作用有关。它可能代表了对干眼症眼表炎症状态的一种病理生理反应。SP-B和SP-C的上调可能代表泪液系统为降低表面张力从而抵消干眼症中泪膜撕裂增加趋势所做的努力。
