重度难治性哮喘患儿的自然杀伤T细胞（iNKT细胞）数量增加。

iNKT cells are increased in children with severe therapy-resistant asthma.

作者信息

Antunes L, Duarte de Souza A P, de Araújo P D, Pinto L A, Jones M H, Stein R T, Pitrez P M

机构信息

Laboratory of Clinical and Experimental Immunology, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil; Infant Center, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil; Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.

出版信息

Allergol Immunopathol (Madr). 2018 Mar-Apr;46(2):175-180. doi: 10.1016/j.aller.2017.05.009. Epub 2017 Dec 24.

DOI:10.1016/j.aller.2017.05.009

PMID:29279262

Abstract

BACKGROUND

Invariant natural killer T (iNKT) cells play complex functions in the immune system, releasing both Th1 and Th2 cytokines. The role of iNKT cells in human asthma is still controversial and never described in severe therapy-resistant asthma in children. The objective of this work was to analyse iNKT frequency in peripheral blood of children with severe therapy-resistant asthma (STRA), compared to children with milder asthma and healthy controls.

METHODS

Children with asthma (n=136) (non-severe and STRA) from a referral centre and healthy controls (n=40) were recruited. Peripheral blood mononuclear cells were isolated, stained with anti-CD3 and anti-iNKT (Vα24Jα18), and analysed through flow cytometry. Atopic status was defined by measuring specific IgE in serum. Airway inflammation was assessed by induced sputum.

RESULTS

Children with asthma presented an increased frequency of CD3iNKT cells (median 0.38% IQR 0.18-1.9), compared to healthy controls (median 0.26% IQR 0.10-0.43) (p=0.025). Children with STRA also showed an increased frequency of iNKT cells (1.5% IQR 1.05-2.73) compared to healthy controls and non-severe asthmatic children (0.35% IQR 0.15-1.6; p=0.002). The frequency of iNKT cells was not different between atopic and non-atopic children. In addition, iNKT cells were not associated with any inflammatory pattern of induced sputum studied.

CONCLUSION

Our data suggests that iNKT cells play a role in paediatric asthma, which is also associated with the severity of disease, but independent of the atopic status.

摘要

背景

不变自然杀伤T（iNKT）细胞在免疫系统中发挥复杂功能，可释放Th1和Th2细胞因子。iNKT细胞在人类哮喘中的作用仍存在争议，且从未在儿童重度难治性哮喘中被描述。本研究的目的是分析重度难治性哮喘（STRA）儿童外周血中iNKT细胞的频率，并与轻度哮喘儿童和健康对照进行比较。

方法

招募了来自转诊中心的哮喘儿童（n = 136）（非重度和STRA）和健康对照（n = 40）。分离外周血单个核细胞，用抗CD3和抗iNKT（Vα24Jα18）染色，并通过流式细胞术进行分析。通过测量血清中的特异性IgE来定义特应性状态。通过诱导痰评估气道炎症。

结果

与健康对照（中位数0.26%，四分位间距0.10 - 0.43）相比，哮喘儿童的CD3iNKT细胞频率增加（中位数0.38%，四分位间距0.18 - 1.9）（p = 0.025）。与健康对照和非重度哮喘儿童（0.35%，四分位间距0.15 - 1.6；p = 0.002）相比，STRA儿童的iNKT细胞频率也增加（1.5%，四分位间距1.05 - 2.73）。特应性和非特应性儿童之间的iNKT细胞频率没有差异。此外，iNKT细胞与所研究的诱导痰的任何炎症模式均无关联。