通过缺失1型马疱疹病毒的被膜蛋白VP22导致即刻早期蛋白ICP4的表达降低。
Decreased expression of the immediate early protein, ICP4, by deletion of the tegument protein VP22 of equine herpesvirus type 1.
作者信息
Okada Ayaka, Suganuma Shota, Badr Yassien, Omatsu Tsutomu, Mizutani Tetsuya, Ohya Kenji, Fukushi Hideto
机构信息
Department of Applied Veterinary Sciences, United Graduated School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
出版信息
J Vet Med Sci. 2018 Feb 20;80(2):311-315. doi: 10.1292/jvms.17-0380. Epub 2017 Dec 26.
VP22 is a major tegument protein of equine herpesvirus type 1 (EHV-1). In the present study, we examined functions of VP22 in EHV-1 replication by viral protein expression analyses in cells infected with the VP22-deficient virus. The expressions of several viral proteins in the cells infected with the VP22-deficient virus were lower than those in the cells infected with the parent virus. One of the weakly expressed proteins was identified as ICP4, which is a major regulatory protein encoded by an immediate early gene of EHV-1. A real-time PCR analysis showed that the mRNA expression of ICP4 was the same in cells infected with the parent and VP22-deficient viruses. Hence, VP22 appears to promote synthesis of ICP4 post-transcriptionally.
VP22是1型马疱疹病毒（EHV-1）的一种主要被膜蛋白。在本研究中，我们通过对感染VP22缺陷病毒的细胞进行病毒蛋白表达分析，研究了VP22在EHV-1复制中的功能。感染VP22缺陷病毒的细胞中几种病毒蛋白的表达低于感染亲本病毒的细胞。其中一种低表达蛋白被鉴定为ICP4，它是由EHV-1的一个立即早期基因编码的主要调节蛋白。实时PCR分析表明，亲本病毒和VP22缺陷病毒感染的细胞中ICP4的mRNA表达相同。因此，VP22似乎在转录后促进ICP4的合成。
Zotero 插件