College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, P. R. China.
School of Chemistry and Environment, Beihang University, Beijing, 100191, P. R. China.
Adv Mater. 2018 Feb;30(6). doi: 10.1002/adma.201701473. Epub 2017 Dec 27.
The utilization of hydrogen peroxide (H O ) cathodic reaction is an ideal approach to develop reliable biosensors that are immune to interferences arising from oxidizable endogenous/exogenous species in biological solutions. However, practical application of such a detection scheme is limited due to the significantly fluctuating oxygen levels in solutions, as oxygen can be reduced at similar potentials. Herein, this limitation is addressed by developing a novel electrode system with superhydrophobicity-mediated air-liquid-solid joint interfaces, which allows the rapid and continuous transport of oxygen from the air phase to the electrode surface and provides a fixed interfacial oxygen concentration. Using cathodic measurement of the enzymatic product H O , the sensing platform is applied to detect glucose, a model analyte, achieving a remarkably high selectivity (≈2% signal modulation due to common biologic interferents), sensitivity (18.56 µA cm mm ), and a dynamic linear range up to 80 × 10 m. The utility of H O reduction reaction at triphase interface to achieve reliable sensing platforms is general, and hence has broad potential in the fields of medical research, clinical diagnosis, and environmental analysis.
过氧化氢 (H O ) 阴极反应的利用是开发可靠的生物传感器的理想方法，因为这种传感器可以免受生物溶液中可氧化的内源性/外源性物质引起的干扰。然而，由于溶液中氧气水平的显著波动（因为氧气在相似的电势下也可以被还原），这种检测方案的实际应用受到限制。为了解决这一限制，本研究开发了一种具有超亲水性介导的气-液-固联合界面的新型电极系统，该系统允许氧气从气相快速连续地传输到电极表面，并提供固定的界面氧气浓度。通过对酶产物 H O 的阴极测量，该传感平台被应用于检测葡萄糖这一模型分析物，实现了极高的选择性（≈2%的信号调制，归因于常见的生物干扰物）、灵敏度（18.56 µA cm mm ）和高达 80×10 m 的动态线性范围。三相界面上 H O 还原反应用于实现可靠传感平台的方法具有普遍性，因此在医学研究、临床诊断和环境分析等领域具有广泛的应用潜力。
