Chen Yangru, Zhang Jun, Ding Zhenyao, Chen Liping, Wang Haili, Zhang Man, Feng Xinjian
College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, China.
Innovation Center for Chemical Science, Soochow University, Suzhou, China.
Front Bioeng Biotechnol. 2022 Apr 28;10:909187. doi: 10.3389/fbioe.2022.909187. eCollection 2022.
Electrocatalytic glucose oxidation is crucial to the development of non-enzymatic sensors, an attractive alternative for enzymatic biosensors. However, due to OH consumption during the catalytic process, non-enzymatic detection generally requires electrolytes having an alkaline pH value, limiting its practical application since biofluids are neutral. Herein, interfacial microenvironment design, we addressed this limitation by developing a non-enzymatic sensor with an air-solid-liquid triphase interface electrodes that synergistically integrates the functions of local alkalinity generation and electrocatalytic glucose oxidation. A sufficiently high local pH value was achieved oxygen reduction reaction at the triphase interface, which consequently enabled the electrochemical oxidation (detection) of glucose in neutral solution. Moreover, we found that the linear detection range and sensitivity of triphase non-enzymatic sensor can be tuned by changing the electrocatalysts of the detection electrode. The triphase electrode architecture provides a new platform for further exploration and promotes practical application of non-enzymatic sensors.
电催化葡萄糖氧化对于非酶传感器的发展至关重要，非酶传感器是酶生物传感器的一种有吸引力的替代方案。然而，由于催化过程中OH的消耗，非酶检测通常需要具有碱性pH值的电解质，这限制了其实际应用，因为生物流体是中性的。在此，通过界面微环境设计，我们开发了一种具有气-固-液三相界面电极的非酶传感器，该电极协同整合了局部碱度产生和电催化葡萄糖氧化的功能，从而解决了这一限制。在三相界面处通过氧还原反应实现了足够高的局部pH值，进而使得葡萄糖能够在中性溶液中进行电化学氧化（检测）。此外，我们发现通过改变检测电极的电催化剂，可以调节三相非酶传感器的线性检测范围和灵敏度。三相电极结构为进一步探索提供了一个新平台，并推动了非酶传感器的实际应用。
