Rashid Neelam, Zafar Muhammad, Ahmad Mushtaq, Malik Khafsa, Haq Ihsan-Ul, Shah Syed Nasar, Mateen Abdul, Ahmed Tauseef
Department of Plant Sciences, Quaid- i- Azam University Islamabad, 45320, Pakistan.
Department of Pharmacy, Quaid-i-Azam University Islamabad, 45320, Pakistan.
Microsc Res Tech. 2018 Mar;81(3):298-307. doi: 10.1002/jemt.22979. Epub 2017 Dec 27.
Seed micromorphology of 12 species of tribe Vicieae (Papilionoidae) representing five genera were examined using Scanning Electron Microscope (SEM). The different seed types were described, illustrated, compared, and their taxonomic importance is discussed. Seeds exhibit great diversity in ultrastructure and a variety of novel morphological features have been determined among and within genera of the tribe. Seeds were characterized by oval to round shape, ovate to oblong outline and striate to papillate ornamentation. Radiate and wavy cell pattern has been observed as a characteristic exomorphological feature. In Vicia sativa, testa cells superficially seem to form peltate hairs. As a result, seed micromorphology with few exceptions showed variation and its taxonomic value was significant in distinguishing taxa at species level. The disparity in shape and density of papillae or protuberances and alignment of testa cells, may possibly give further insight at intraspecific level within tribe. The findings also illustrated that the use of SEM in seed morphology can reveal hidden morphological affinities among species and showed potential in delimitation of Vicieae members generally at tribe, genus, and particularly at species level.
利用扫描电子显微镜（SEM）对蝶形花科野豌豆族5个属的12个物种的种子微观形态进行了研究。描述、阐释并比较了不同的种子类型，并讨论了它们在分类学上的重要性。种子在超微结构上表现出极大的多样性，并且在该族的属间和属内确定了多种新的形态特征。种子的特征为椭圆形至圆形，卵形至长圆形轮廓，以及具条纹至具乳头状纹饰。辐射状和波浪状细胞模式已被观察到是一种特征性的外部形态特征。在野豌豆中，种皮细胞表面似乎形成盾状毛。因此，除少数例外，种子微观形态表现出变异，其在分类学上的价值对于区分物种水平的分类单元具有重要意义。乳头或突起的形状和密度以及种皮细胞排列的差异，可能会在该族的种内水平上提供进一步的见解。研究结果还表明，在种子形态学中使用扫描电子显微镜可以揭示物种间隐藏的形态学亲缘关系，并在野豌豆族成员的界定中，总体上在族、属以及特别是在物种水平上显示出潜力。
