Department of Obstetrics and Gynecology, University of Toyama, Toyama City, Japan.
Clinical Laboratory Center, Toyama University Hospital, Toyama City, Japan.
Am J Reprod Immunol. 2018 Feb;79(2). doi: 10.1111/aji.12807. Epub 2017 Dec 27.
To investigate whether amniotic fluid (AF) "sludge" in patients with preterm labor (PTL) with intact membranes is related to intra-amniotic infection or inflammation.
105 PTL patients before 29 weeks' gestation were enrolled. AF "sludge" was evaluated by transvaginal sonography. Microorganisms were identified in AF by our newly established PCR method using a eukaryote-made thermostable DNA polymerase.
AF "sludge" was present in 18.1% (19/105) of patients. The results obtained in the AF "sludge" group vs the no "sludge" group were as follows: (i) a similar positive rate of microorganisms in AF by PCR, 31.6% (6/19) vs 38.4% (33/86); (ii) a higher level of AF interleukin-8, 15.2 (0.2-381.5) ng/mL vs 5.8 (0.1-413.7) ng/mL; P = .005); and (3) a higher frequency of histological chorioamnionitis, 52.6% (10/19) vs 23.3% (20/86); P = .010.
The presence of AF "sludge" is related to intra-amniotic inflammation with or without microorganisms.
研究在胎膜完整的早产（PTL）患者中，羊水（AF）“碎屑”是否与羊膜内感染或炎症有关。
纳入了 105 名 29 周前的 PTL 患者。通过经阴道超声检查评估 AF“碎屑”。使用我们新建立的 PCR 方法，使用真核生物耐热 DNA 聚合酶鉴定 AF 中的微生物。
18.1%（19/105）的患者存在 AF“碎屑”。AF“碎屑”组与无“碎屑”组的结果如下：（i）PCR 检测 AF 中微生物的阳性率相似，31.6%（6/19）与 38.4%（33/86）；（ii）AF 白细胞介素-8 水平较高，15.2（0.2-381.5）ng/mL 与 5.8（0.1-413.7）ng/mL；P=0.005）；（3）组织学绒毛膜羊膜炎的发生率较高，52.6%（10/19）与 23.3%（20/86）；P=0.010。
AF“碎屑”的存在与羊膜内炎症有关，无论是否存在微生物。
