Bioconjug Chem. 2018 Mar 21;29(3):672-679. doi: 10.1021/acs.bioconjchem.7b00620. Epub 2018 Jan 19.
Functional analysis of lymphocytes is important for development of vaccines and diagnosis/treatment of various immune-related diseases. In this review, we describe multifunctional microwell arrays that enable functional analysis of lymphocytes at the single cell level. We first discuss key parameters for microwell array design. Then, we describe how different types of multifunctional microwell arrays were developed for various applications, including live cell imaging of lymphocyte activation, proliferation, and differentiation, and analyses of effector functions such as cytokine secretion and target cell lysis. Incorporation of novel surface chemistries and functional materials into microwell arrays for enhancing sensing capabilities will widen applications of this technology. Multifunctional microwell arrays will be a powerful tool for the development of novel therapeutics against immune-related diseases, in particular, for cancer immunotherapy.
淋巴细胞的功能分析对于疫苗的开发和各种免疫相关疾病的诊断和治疗非常重要。在这篇综述中，我们描述了多功能微井阵列，这些阵列可以实现单细胞水平的淋巴细胞功能分析。我们首先讨论了微井阵列设计的关键参数。然后，我们描述了如何为各种应用开发不同类型的多功能微井阵列，包括淋巴细胞激活、增殖和分化的活细胞成像，以及细胞因子分泌和靶细胞裂解等效应功能的分析。将新型表面化学和功能材料纳入微井阵列以增强传感能力，将拓宽该技术的应用范围。多功能微井阵列将成为开发针对免疫相关疾病的新型治疗方法的有力工具，特别是癌症免疫疗法。
