酶促产生硫化氢的时间分辨发光检测：基于镧系元素探针的设计、合成及应用
Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide: Design, Synthesis, and Application of a Lanthanide-Based Probe.
作者信息
Yao Yao, Delgado-Rivera Loruhama, Samareh Afsari Hamid, Yin Liang, Thatcher Gregory R J, Moore Terry W, Miller Lawrence W
机构信息
Department of Chemistry, University of Illinois at Chicago , 845 W. Taylor Street, MC 111, Chicago, Illinois 60607, United States.
Department of Medicinal Chemistry and Pharmacognosy, UICentre for Drug Discovery, University of Illinois at Chicago , Chicago, Illinois 60612, United States.
出版信息
Inorg Chem. 2018 Jan 16;57(2):681-688. doi: 10.1021/acs.inorgchem.7b02533. Epub 2017 Dec 27.
Abstract
Hydrogen sulfide (HS) is now recognized as an important gaseous transmitter that is involved in a variety of biological processes. Here, we report the design and synthesis of a luminescent lanthanide biosensor for HS, LP2-Cu(II)-Ln(III), a heterobinuclear metal complex that uses Cu(II) decomplexation to control millisecond-scale-lifetime-Tb(III)- or Eu(III)-emission intensity. LP2-Cu(II)-Ln(III) responded rapidly, selectively, and with high sensitivity to aqueous HS. The probe's potential for biological applications was verified by measuring the HS generated by the slow-releasing chemical-sulfide-donor GYY4147, by cystathionine γ-lyase (CSE), and by NaS-stimulated HeLa cells.
摘要
硫化氢（HS）现已被公认为是一种参与多种生物过程的重要气体递质。在此，我们报告了一种用于HS的发光镧系生物传感器LP2-Cu(II)-Ln(III)的设计与合成，它是一种异双核金属配合物，利用Cu(II)的解络合作用来控制毫秒级寿命的Tb(III)或Eu(III)发射强度。LP2-Cu(II)-Ln(III)对水溶液中的HS反应迅速、选择性高且灵敏度高。通过测量缓释化学硫化物供体GYY4147、胱硫醚γ-裂合酶（CSE）以及NaS刺激的HeLa细胞产生的HS，验证了该探针在生物应用方面的潜力。
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